Nuclear targeting peptide-modified, DOX-loaded, PHBV nanoparticles enhance drug efficacy by targeting to Saos-2 cell nuclear membranes
Yazarlar (5)
Ayla Şahin Middle East Technical University
Dr. Öğr. Üyesi Gözde EKE ÇEVİK Kırşehir Ahi Evran Üniversitesi, Türkiye
Dr. Öğr. Üyesi Arda Büyüksungur Ankara Üniversitesi, Türkiye
Vasıf Nejat Hasırcı Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
Nesrin Hasırcı Yakın Doğu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (Q2) | ||
| Dergi ISSN | 0920-5063 Dergi Bilgileri (2018) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2018 |
| Cilt / Sayı / Sayfa | 29 / 5 / 507–519 | DOI | 10.1080/09205063.2018.1423812 |
| UAK Araştırma Alanları |
Mikroteknolojiler
|
||
| Özet |
| The aim of this study was to target nano sized (266 +/- 25nm diameter) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) particles carrying Doxorubicin (DOX), an anticancer agent, to human osteosarcoma cells (Saos-2). A nuclear targeting molecule (Nuclear Localization Signal, NLS), a 17 a.a. peptide, was attached onto the doxorubicin loaded nanoparticles. NLS conjugated nanoparticles surrounded the cell nuclei, but did not penetrate them. Free doxorubicinand doxorubicin loadednanoparticles entered the cytoplasm and were evenly distributed within the cytoplasm. The localization of the NLS-targetedparticles around the nuclear membrane caused a significantly higher decrease in the cancer cell numbers due to apoptosis or necrosis than the untargeted and free doxorubicin formulations showing the importance of targeting the nanoparticles to the nuclear membrane in the treatment of cancer. |
| Anahtar Kelimeler |
| PHBV | biodegradable nanoparticles | nuclear drug delivery | doxorubicin | nuclear localization signal |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 16 |