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An estradiol releasing, proangiogenic hydrogel as a candidate material for use in soft tissue interposition     
Yazarlar
Naşide Mangır
Hacettepe Üniversitesi, Türkiye
Dr. Öğr. Üyesi Gözde EKE ÇEVİK Dr. Öğr. Üyesi Gözde EKE ÇEVİK
Kırşehir Ahi Evran Üniversitesi, Türkiye
Nesrin Hasırcı
Yakın Doğu Üniversitesi, Türkiye
Christopher Chapple
Vasıf Nejat Hasırcı
Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
Sheila Macneil
Özet
Introduction and Objectives Soft tissue interposition (STI) using local and/or regional flaps is often necessary in urogenital reconstruction to stimulate wound healing and prevent recurrence. Harvesting STI flaps can cause donor site morbidity and may not be available in some patients. In this study, we designed estradiol (E2) releasing hydrogel that could be used as an alternative to a STI flap and to investigate its ability to stimulate tissue production and angiogenesis. Materials and Methods A hydrogel was constructed by crosslinking a solution of estradiol, methacrylated gelatin (15%, w/v), and methacrylated hyaluronic acid (1%, w/v). The release of estradiol was measured using a UV-spectrophotometer (lambda(max) = 220 nm). Angiogenesis was evaluated by an ex ovo chicken embryo chorioallantoic membrane (CAM) assay. Results Estradiol was gradually released from the hydrogel over 21 days. The hydrogels could be easily manipulated with surgical forceps without any deformation. The hydrogels significantly increased collagen production of human dermal fibroblasts (HDFs). Scanning electron microscopic examination demonstrated that HDFs produced significantly more extracellular matrix (ECM) on estradiol releasing hydrogels compared with the controls. Estradiol releasing hydrogels doubled the number of blood vessels growing toward the hydrogel compared with the controls (vasculogenic index, 59.6 [+/- 6.4] and 25.6 [+/- 4.0], respectively; [P < 0.05]). Conclusion We present a proangiogenic, degradable hydrogel that can be used as an off-the-shelf available substitute to traditional STI flaps. This is achieved by using estradiol as a potent stimulator of new tissue production and new blood vessel formation.
Anahtar Kelimeler
estradiol | hydrogel | tissue interposition flap | vascularization | vesicovaginal fistula
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı NEUROUROLOGY AND URODYNAMICS
Dergi ISSN 0733-2467
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili Türkçe
Basım Tarihi 06-2019
Cilt No 38
Sayı 5
Sayfalar 1195 / 1202
Doi Numarası 10.1002/nau.23971
Makale Linki http://dx.doi.org/10.1002/nau.23971