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The bioactive efficiency of ultrasonic extracts from acorn leaves and green walnut husks against Bacillus cereus: a hybrid approach to PCA with the Taguchi method       
Yazarlar
Dr. Öğr. Üyesi Göktürk ÖZTÜRK Dr. Öğr. Üyesi Göktürk ÖZTÜRK
Kırşehir Ahi Evran Üniversitesi, Türkiye
Ahmet Evren Yetiman
Erciyes Üniversitesi, Türkiye
Mahmut Doğan
Erciyes Üniversitesi, Türkiye
Özet
The objective of this study was to investigate the usefulness of the hybrid approach using the Taguchi method (TM) and principal component analysis (PCA) in determining the optimum conditions for ultrasonic extracts of acorn leaves and green walnut husks, which potentially demonstrate the best bioactive efficiency against Bacillus cereus. First, an L36 (21 × 34) mixed level orthogonal array design was implemented, consisting of five factors: extract type, temperature, time, solvent and concentration, respectively. Also, the total phenolic content, antiradical activity, and antibiogram analyses were investigated by design, and signal-to-noise (S/N) ratios were calculated for each trial. An analysis of variance (ANOVA) was performed using S/N ratios to estimate the effects of the measured parameters and their interactions for a single-objective TM. Following that, PCA was used to normalize the S/N ratios for each response to calculate the multi-response performance index for measuring the effects of factors on all responses. In this study, the expected optimal factor levels for each experiment were found to be different for a single-objective TM and inadequate for interpreting all responses simultaneously. The results of the study showed that the optimal conditions for all responses with PCA-based TM was found to be a 5% concentration of acorn leave extract and 50% acetone at 60 °C for 60 min, with 136.96 mg g–1 gallic acid equivalent, 90.75% inhibited 2,2-diphenyl-1-picrylhydrazyl and 14.33 mm inhibition zone against B. cereus.
Anahtar Kelimeler
Antimicrobial activity | Bacillus cereus | Hybrid approach | Phenolic | Principal component analysis | Taguchi method
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
Dergi ISSN 2193-4126
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili İngilizce
Basım Tarihi 06-2019
Cilt No 13
Sayı 2
Sayfalar 1257 / 1268
Doi Numarası 10.1007/s11694-019-00041-7
Makale Linki https://link.springer.com/article/10.1007/s11694-019-00041-7