| Yazarlar |
Ozan Luay Abbas
Ahi Evran Üniversitesi, Türkiye |
|
Yunus Kasım Terzi
Başkent Üniversitesi, Türkiye |
|
Orhan Özatik
Dumlupınar Üniversitesi, Türkiye |
|
Fikriye Yasemin Özatik
Dumlupınar Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Gamze TURNA SALTOĞLU
Ahi Evran Üniversitesi, Türkiye |
|
Rukiye Nar
Ahi Evran Üniversitesi, Türkiye |
|
Ahmet Musmul
Eskişehir Osmangazi Üniversitesi, Türkiye |
| Özet |
| Background: Smoke of cigarettes, and specifically nicotine, has been shown to diminish pedicled transverse rectus abdominis musculocutaneous (TRAM) flap survival. Considering that Notch signalling through its ligand Delta-like 4 (Dll4) functions as anti-angiogenic factor by inhibiting the pro-angiogenic effects of vascular endothelial growth factor (VEGF), it is hypothesised that inhibition of the Notch would promote angiogenesis and increase TRAM flap survival in rats submitted to nicotine. Methods: Twenty rats were treated with nicotine for 28 days preoperatively. Thereafter, a pedicled TRAM flap was created in all animals. The Notch inhibitor N-[N-(3,5-difluorophenacetyl)-1-alanyl]-S-phenylglycine-t-butyl-ester was administered in animals of the treatment group. Animals in the control group were given the same amount of solvent. Five days after the surgery, viable flap areas were determined. Skin samples were evaluated for VEGF and Dll4 mRNA levels. Immunohistochemical analysis was used for the assessment of endothelial Dll4 expression. Vascular density was determined histologically. Plasma levels of VEGF and Dll4 were measured. Results: A significant improvement in TRAM flap surviving area was observed in the treatment group (53.5014.25%) compared with the controls (32.20 +/- 9.15%). Immunohistochemical analysis revealed a significant increase in the number of Dll4 stained vessels in animals of the treatment group (9.2 +/- 1.6) in comparison with the controls (5.7 +/- 1.9). VEGF mRNA levels (0.22 +/- 0.08) in the treatment group were significantly lower than those in the control group (0.36 +/- 0.09). Conclusion: Notch inhibition significantly improved TRAM flap survival in animals exposed to nicotine by promoting VEGF-induced angiogenesis. |
| Anahtar Kelimeler |
| Notch | Dll4 | TRAM | nicotine | survival | angiogenesis |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF PLASTIC SURGERY AND HAND SURGERY |
| Dergi ISSN | 2000-656X |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2017 |
| Cilt No | 51 |
| Sayı | 6 |
| Sayfalar | 405 / 413 |
| Doi Numarası | 10.1080/2000656X.2017.1285784 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.1080/2000656X.2017.1285784 |
| Atıf Sayıları | |
| WoS | 1 |
