| Yazarlar (3) |
Kudret Dilsad Barut
|
Prof. Dr. Fatma Filiz ARI
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Ayşe Filiz Öner
Hacettepe Üniversitesi, Türkiye |
| Özet |
| The development of efficient and stable carriers for the delivery of DNA to the body is becoming an increasingly important issue in the fields of gene therapy and yaccination. The present study was designed to prepare an emulsion-based gene delivery system. Oil-in-water emulsions containing cetyltrimethylammonium bromide (CTAB) as a cationic surfactant and Pluronic F-68 as a nonionic cosurfactant were formulated and their physical characteristics were investigated. The cationic emulsion, referred to as B13d, containing a liquid crystal phase, was found to be stable and suitable for parenteral application. Complex formation between pGL3 plasmid DNA and B13d emulsion achieved at the pDNA:CTAB ratio of 1:2.74 ($mu g$/nmol) was demonstrated using the gel retardation and SYBR Green I displacement assays. Particle sizes of the freshly prepared empty and pDNA-associated B13d emulsions were about 350 nm. Zeta potentials of empty and pDNA loaded emulsions were +45.3 mV and +43.7 mV, respectively. The sensitivity of pDNA/emulsion complexes to endonuclease digestion was determined and complexes were found to be highly effective for protecting pDNA from DNase I attack. The final in vitro stability analysis carried out in the presence of human serum revealed that the super-coiled pDNA was observable even after 24 h of incubation. The physical characteristics and serum resistant properties of the complexes suggest that B13d emulsion could be an efficient pDNA carrier system for gene and/or immunogene delivery. |
| Anahtar Kelimeler |
| Cationic emulsions | Cetyltrimethylammonium bromide (CTAB) | Gene delivery | Plasmid DNA |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | TURKISH JOURNAL OF CHEMISTRY |
| Dergi ISSN | 1300-0527 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2005 |
| Cilt No | 29 |
| Sayı | 1 |
| Sayfalar | 27 / 40 |
