Comparison of Three Methods of DNA Extraction from Parachipteria Willmanni Acari Oribatida Collected in Turkey
Yazarlar (10)
Sedat Per Bozok Üniversitesi, Türkiye
Prof. Dr. Fahriye ERCAN Ahi Evran Üniversitesi, Türkiye
Emine Çağlar Türkiye
Gıyasettin Demirhan Türkiye
Sadettin Kirazcı Türkiye
Ayda Karaca Türkiye
Nigar Küçükkubaş Türkiye
Elif Nursel Özmert Türkiye
Erdem Karabulut Türkiye
Mehmet Mesut Çelebi Türkiye
| Makale Türü | Özgün Makale (Diğer hakemli uluslarası dergilerde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Biotechnology Research | ||
| Dergi Tarandığı Indeksler | ADVANCE SCIENCE INDEX | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2015 |
| Cilt / Sayı / Sayfa | 1 / 4 / 16–20 | DOI | – |
| Özet |
| We compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocol that obtained from Parachipteria willmanni van der Hammen, 1952 mite specimens preserved in ethanol. The aim of the study is to find the most efficient protocol for obtaining DNA from Parachipteria willmanni. All methods are successful in isolating DNA from P. willmanni, but among these three methods, maximum amount of DNA is obtained with Chelex-method. DNAs obtained by these three methods were successfully applied to RAPD-PCR. |
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