| Yazarlar (5) |
Dr. Öğr. Üyesi Esra İLHAN AYIŞIĞI
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Gözde Budak
|
|
Melih Soner Çeliktaş
|
|
Canan Sevimli Gür
İzmir Katip Çelebi Üniversitesi, Türkiye |
|
Özlem Yeşil Çeliktaş
Ege Üniversitesi, Türkiye |
| Özet |
| Cereal grain-derived protein hydrolysates exert beneficial effects for human health, thus utilization of by-products with high protein content has drawn attention. In this study, hot water extraction of rice husk has been optimized to obtain protein hydrolysate with the highest anticancer activity. The optimum protein value was obtained as 2.40 g/L corresponding to 43.2 g protein/kg dried rice husk under the conditions of 60 degrees C, 2.0 mL/min flow rate and pH 10.0. The protein hydrolysate was encapsulated with chitosan, where the mean particle size of protein hydrolysate (0.3 %) loaded chitosan nanoparticles was 256.4 +/- 33. 4 nm with 89 % encapsulation efficiency and a 65% release at the end of 6 days. Cytotoxicity assays showed that the lowest cell viabilities have been achieved with A549 and MCF7 cells with IC50 values of 1.98 and 3.58 mu g/mL, respectively, whereas nuclear fragmentation and apoptotic bodies were observed through Hoechst 33342 staining. The cytotoxic effect might be associated with the wide variety of peptide/protein subunits ranging from 10 kDa to more than 180 kDa in the protein hydrolysate of rice husk. (C) 2021 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved. |
| Anahtar Kelimeler |
| Chitosan nanoparticle | Cytotoxicity | Hot water extraction | Peptide | Rice husk |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY |
| Dergi ISSN | 1226-086X Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | Türkçe |
| Basım Tarihi | 11-2021 |
| Cilt No | 103 |
| Sayı | 1 |
| Sayfalar | 381 / 391 |
| Doi Numarası | 10.1016/j.jiec.2021.08.006 |
| Makale Linki | http://dx.doi.org/10.1016/j.jiec.2021.08.006 |
| Atıf Sayıları | |
| WoS | 19 |
| SCOPUS | 23 |
| Google Scholar | 25 |
