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Can functional near infrared spectroscopy (fNIRS) be used for the estimation of the numbers and types of muscle fibres in sheep?   
Yazarlar (8)
Prof. Dr. Emre ŞİRİN Prof. Dr. Emre ŞİRİN
Türkiye
Ömer Saylı
Zafer Ulutaş
Niğde Ömer Halisdemir Üniversitesi, Türkiye
Uğur Şen
Ondokuz Mayıs Üniversitesi, Türkiye
Sinem Burcu Erdoğan
Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
Yüksel Aksoy
Eskişehir Osmangazi Üniversitesi, Türkiye
Ata Akın
Acıbadem Mehmet Ali Aydınlar Üniversitesi, Türkiye
Mehmet Kuran
Ondokuz Mayıs Üniversitesi, Türkiye
Devamını Göster
Özet
A novel technique, fNIRS, have succesfully been used for the measurement of oxidative metabolism of muscles in human (1). This study is aimed to investigate whether fNIRS can be used to estimate the numbers and metabolic types of muscle fibres in lambs by measuring oxidative metabolism of semimembranosus (SM) muscle in vivo in sheep. fNIRS is a novel, non-invasive, rapid and inexpensive method that uses light sources and detectors placed on the skin surface to probe the oxygen metabolism of the underlying tissues. A day before slaughter, NIROXCOPE 301, a continuous wave fNIRS device was used to measure the changes in muscle hemodynamic response to electrical stimulation in 120 (n= 16) or 165 (n= 8) days old lambs. The fNIRS probe was placed on the skin above the muscle and the measurements were acquired during full ischemia with two different stimulation frequencies (40 Hz vs. 150 Hz). NIROXCOPE 301 system provides local concentration changes of deoxyhemoglobin, oxyhemoglobin and oxygen consumption in units of µmol/s. After slaughter, SM muscle was isolated and frozen muscle samples were subjected to ATPase and SDH staining to determine contractile and metabolic types of muscle fibres and the number of fibres in an area.There were significant positive correlations between the proportion of oxidative muscle fibres in SM muscle and the decrease in oxygen consumption (µmol/s) in response to electrical stimulation on day 120 (r= 0.58; P< 0.05) and on day 164 (r= 0.69; P= 0.06). Negative correlations were observed for proportions of type I or type IIB muscle fibres and decrease in oxygen consumption …
Anahtar Kelimeler
Bildiri Türü Tebliğ/Bildiri
Bildiri Alt Türü Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum)
Bildiri Niteliği Alanında Hakemli Uluslararası Kongre/Sempozyum
Bildiri Dili İngilizce
Kongre Adı Cost Action 925
Kongre Tarihi 07-11-2007 / 07-11-2007
Basıldığı Ülke
Basıldığı Şehir
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 2

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