| Yazarlar (2) |
Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| The enzyme of RNA polymerase is consisted of two subunits. One of these is core RNA polymerase consisting of five subunits (αI, αII, β, β’and ω) which is responsible for catalytic activity. The second subunit is known as specific σ factor and it is responsible for recognizing promoters with specific DNA sequences. The sigma factor σ28 is responsible for expression of related genes in flagellar biosynthesis and chemotaxis in many bacteria such as Escherichia coli, Pseudomonas aeruginosa and, Bacillus subtilis. In this study, we cloned the sigma factor (fliA) of RNA polymerase from two thermophilic bacteria (Geobacillus kaustophilus and Anoxybacillus flavithermus) and, transformed into a fliA mutant of E. coli YK4104 under expression of T7 promoter included in pET-(28a)+ plasmid vector. Interestingly, we showed that the fliA genes from G. kaustophilus and A. flavithermus restored the motility to the E. coli mutant which is a mesophilic bacterium. This indicates that G. kaustophilus and A. flavithermus σ28 protein is able to bind to the E. coli core RNA polymerase and, participate in the complex interactions to initiate the transcription of the flagellar units in vivo. These results suggest that the binding ability of thermofilic σ28 to the core RNA polymerase is not dependent on high temperature. |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Tam Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | 2nd International Congress on the World of Technology and Advanced Materials |
| Kongre Tarihi | 28-09-2016 / 02-10-2016 |
| Basıldığı Ülke | |
| Basıldığı Şehir |
