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Molecular Characterization of Antibiotic Resistant Escherichia coli Strains Isolated from Tap and Spring Waters in a Coastal Region in Turkey    
Yazarlar (5)
Osman Birol Özgümüş
Recep Tayyip Erdoğan Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Şengül Alpay Karaoğlu
Recep Tayyip Erdoğan Üniversitesi, Türkiye
Cemal Sandallı
Recep Tayyip Erdoğan Üniversitesi, Türkiye
Prof. Dr. Ali SEVİM Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
A hundred and seventeen antibiotic-resistant Escherichia coli strains were isolated from public tap and spring waters which were polluted by fecal coliforms. There were no significant differences between two water sources as to the coliform pollution level (p> 0.05). All E. coli isolates were detected to be resistant to one or more antibiotics tested. Nearly 42% of the isolates showed multiresistant phenotype. Three (2.5%) of these isolates contained class 1 integron. Sequencing analysis of variable regions of the class 1 integrons showed two gene cassette arrays, dfr1-aadA1 and dhfrA17-aadA5. Resistance to ampicillin, tetracycline or trimethoprim-sulfamethoxazole was transferable according to the results of conjugation experiments. The rate of tetracycline resistance was 15%. tet (A)-mediated tetracycline resistance was widespread among tetracycline-resistant E. coli isolates. Genotyping by BOX-polymerase chain reaction (BOX-PCR) showed that some of the strains were epidemiologically related. This is the first report on the prevalence and characterization of class 1 integron-containing E. coli isolates of environmental origin in Turkey.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı The Journal Of Microbiology
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2007
Cilt No 45
Sayı 5
Sayfalar 379 / 387
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 89

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