img
Determination of the bacterial flora as a microbial control agent of Toxoptera aurantii Homoptera Aphididae     
Yazarlar
Prof. Dr. Elif SEVİM Prof. Dr. Elif SEVİM
Ahi Evran Üniversitesi, Türkiye
Ömer Çelebi
Prof. Dr. Ali SEVİM Prof. Dr. Ali SEVİM
Ahi Evran Üniversitesi, Türkiye
Özet
Toxoptera aurantii (Homoptera: Aphididae) is one of the most important pests of many agricultural plants such as camellia, cocoa and coffee worldwide. The culturable bacterial flora of the pest was determined to find new microbes that can be used as biocontrol agent against T. aurantii. A total of six bacteria were isolated and identified by a variety of morphological, physiological, biochemical and molecular tests. In addition, an approximately 1,150 bp fragment of the 16S rRNA gene region was sequenced to verify isolate identification. According to the identification studies, the isolates were identified as Bacillus tequilensis Cb1, Chryseobacterium stagni Cb2, Pseudomonas flourescens Cb3, Rahnella aquatilis Cb4, Staphylococcus sp. Cb5 and Cb6. Pathogenicity of the bacterial isolates were carried out against the last instar nymphs of T. aurantii. The highest activity was obtained from Pseudomonas flourescens Cb3 with 50% mortality within 10 days after application (p<0.05). Mortalities of other treatments ranged from 6.6 to 20%. The results presented here show that Pseudomonas flourescens Cb3 appears to be a significant candidate as a possible biocontrol agent against T. aurantii and should be beneficial in the future biocontrol programs of the pest. © 2012 Versita Warsaw and Springer-Verlag Wien.
Anahtar Kelimeler
bacterial flora | black citrus aphid | pathogenicity | Pseudomonas flourescens
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Biologia
Dergi ISSN 1336-9563
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 01-2012
Cilt No 67
Sayı 2
Sayfalar 397 / 404
Doi Numarası 10.2478/s11756-012-0022-0
Makale Linki http://www.degruyter.com/view/j/biolog.2012.67.issue-2/s11756-012-0022-0/s11756-012-0022-0.xml