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Isolation and characterization of Beauveria and Metarhizium spp. from walnut fields and their pathogenicity against the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)     
Yazarlar
Songül Gürlek
Prof. Dr. Ali SEVİM Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Fikriye Milletli Sezgin
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
Entomopathogenic fungi (EPFs) play an important role for regulating insect pest populations, as they exist in many different ecosystems. Within these fungi, Beauveria and Metarhizium spp. genera include species that are the most commercially important. The aim of this study was to determine the diversity and distribution of Beauveria and Metarhizium spp. in walnut fields of Kırşehir, Turkey, and to evaluate their pathogenicity against Cydia pomonella (L.) (Lepidoptera: Tortricidae). To perform this, 90 soil samples were collected from walnut fields where Beauveria and Metarhizium spp. were isolated from these soils, using selective media. The isolated 40 fungi were characterized based on their morphological and molecular characteristics including Bloc and β-tubulin gene sequences. Also, eight selected fungi were tested against C. pomonella larvae under laboratory conditions. The fungal isolates were identified as Beauveria pseudobassiana (15), B. bassiana (12), Metarhizium robertsii (11), and M. brunneum (13). M. brunneum ELA-38 caused 83% mortality within 2 weeks after application of 1 × 108 conidia/ml. Consequently, Beauveria and Metarhizium spp. are the common component of the soils collected from walnut fields and some of fungi obtained from this work might be beneficial in the future biological control programs of C. pomonella.
Anahtar Kelimeler
Codling moth | Cydia pomonella | Entomopathogenic fungi | Pathogenicity | Walnut
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Egyptian Journal of Biological Pest Control
Dergi ISSN 1110-1768
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 12-2018
Cilt No 28
Sayı 1
Sayfalar 1 / 6
Doi Numarası 10.1186/s41938-018-0055-y
Makale Linki https://ejbpc.springeropen.com/articles/10.1186/s41938-018-0055-y