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Molecular Characterization of Gram Negative Isolated from Walnut (Juglans regia L.) Rhizospheric Soil     
Yazarlar
Prof. Dr. Belgin ERDEM Prof. Dr. Belgin ERDEM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Ali SEVİM Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
In this study, 10 Gram negative bacteria isolated from the rhizosphere soil of walnut (Juglans regia) orchard in the Kırşehir (Central Anatolia of Turkey) were identified at species level by classical and molecular methods. It was determined that 99% of phylogenetic similarities with bacterial isolates were closely related to the isolated Gram negative bacterial isolates. Ten isolates were morpho-biochemically and molecularly characterized and on the basis of 16S rDNA sequencing were identified as 3 Enterobacter cloacae, 3 Enterobacter ludwigii, 2 Escherichia coli, 1 Enterobacter hormaechei and 1 Plesiomonas shigelloides. Gram negative bacteria showed enzymes activities. Except for P. shigelloides and E. coli, other Gram negative bacteria showed lipase, protease, cellulase, amylase, gelatinase and pectinase activities at 4 °C and 28 °C. As a result, the current research suggests that strains isolated from walnut rhizosphere soil can be used as bio-fertilizing and bio-control agents.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Uluslararası alan indekslerindeki dergilerde yayımlanan tam makale
Dergi Adı Black Sea Journal of Engineering and Science
Dergi ISSN 2619-8991
Dergi Tarandığı Indeksler Index Copernicus
Makale Dili Türkçe
Basım Tarihi 02-2022
Doi Numarası 10.34248/bsengineering.957791
Makale Linki http://dx.doi.org/10.34248/bsengineering.957791