Molecular Characterization of Gram Negative Isolated from Walnut (Juglans regia L.) Rhizospheric Soil
Yazarlar (3)
Prof. Dr. Belgin ERDEM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale)
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| Dergi Adı | Black Sea Journal of Engineering and Science | ||
| Dergi ISSN | 2619-8991 | ||
| Dergi Tarandığı Indeksler | Index Copernicus | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2022 |
| Cilt / Sayı / Sayfa | 5 / 2 / 54–61 | DOI | 10.34248/bsengineering.957791 |
| Makale Linki | http://dx.doi.org/10.34248/bsengineering.957791 | ||
| Özet |
| In this study, 10 Gram negative bacteria isolated from the rhizosphere soil of walnut (Juglans regia) orchard in the Kırşehir (Central Anatolia of Turkey) were identified at species level by classical and molecular methods. It was determined that 99% of phylogenetic similarities with bacterial isolates were closely related to the isolated Gram negative bacterial isolates. Ten isolates were morpho-biochemically and molecularly characterized and on the basis of 16S rDNA sequencing were identified as 3 Enterobacter cloacae, 3 Enterobacter ludwigii, 2 Escherichia coli, 1 Enterobacter hormaechei and 1 Plesiomonas shigelloides. Gram negative bacteria showed enzymes activities. Except for P. shigelloides and E. coli, other Gram negative bacteria showed lipase, protease, cellulase, amylase, gelatinase and pectinase activities at 4 °C and 28 °C. As a result, the current research suggests that strains isolated from walnut rhizosphere soil can be used as bio-fertilizing and bio-control agents. |
| Anahtar Kelimeler |
| Gram negetive bacteria | 16S rRNA sequence | Juglans regia L. | Rhizosphere |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |