| Yazarlar (5) |
Dr. Öğr. Üyesi Ekin ÇELİK
Ahi Evran Üniversitesi, Türkiye |
Cem Bayram
Hacettepe Üniversitesi, Türkiye |
|
Rümeysa Akçapınar
|
|
Mustafa Türk
Kırıkkale Üniversitesi, Türkiye |
|
Emir Baki Denkbaş
Hacettepe Üniversitesi, Türkiye |
| Özet |
| Peptide based hydrogels gained a vast interest in the tissue engineering studies thanks to great superiorities such as biocompatibility, supramolecular organization without any need of additional crosslinker, injectability and tunable nature. Fmoc-diphenylalanine (FmocFF) is one of the earliest and widely used example of these small molecule gelators that have been utilized in biomedical studies. However, Fmoc-peptides are not feasible for long term use due to low stability and weak mechanical properties at neutral pH. In this study, Fmoc-FF dipeptides were mechanically enhanced by incorporation of alginate, a biocompatible and absorbable polysaccharide. The binary hydrogel is obtained via molecular self-assembly of FmocFF dipeptide in alginate solution followed by ionic crosslinking of alginate moieties with varying concentrations of calcium chloride. Hydrogel characterization was evaluated in terms of morphology, viscoelastic moduli and diffusional phenomena and the structures were tested as 3D scaffolds for bovine chondrocytes. In vitro evaluation of scaffolds lasted up to 14days and cell viability, sulphated glycosaminoglycan (sGAG) levels, collagen type II synthesis were determined. Our results showed that alginate incorporation into FmocFF hydrogels leads to better mechanical properties and higher stability with good biocompatibility. |
| Anahtar Kelimeler |
| 3D cell culture | Alginate | FmocFF | Cartilage | Scaffold | Hydrogel |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Materials Science and Engineering: C |
| Dergi ISSN | 0928-4931 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2016 |
| Cilt No | 66 |
| Sayfalar | 221 / 229 |
| Doi Numarası | 10.1016/j.msec.2016.04.084 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S0928493116303927 |
| Atıf Sayıları | |
| WoS | 51 |
| Google Scholar | 71 |
