| Yazarlar (11) |
Eda Yalçın
|
|
Göknur Kara
Ordu Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Ekin ÇELİK
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Ferda Pınarlı
|
|
Guleser Saylam
|
|
Ceren Sucularlı
Hacettepe Üniversitesi, Türkiye |
|
Serhat Ozturk
|
|
Esın Yılmaz
|
|
Ömer Bayır
|
|
Mehmet Hakan Korkmaz
Ankara Yıldırım Beyazıt Üniversitesi, Türkiye |
|
Emir Baki Denkbaş
Başkent Üniversitesi, Türkiye |
| Özet |
| Small interfering RNA (siRNA)-based gene silencing strategy has high potential on suppressing specific molecular targets, involved in cancer progression. However, the lack of an effective nanocarrier system that safely delivers siRNA to its target still limits the clinical applications of siRNA. This study aimed to develop albumin-sericin nanoparticles (Alb-Ser NPs) as a novel siRNA delivery system for laryngeal cancer treatment. Nanoparticle formulations composed of albumin and sericin at different ratios (1:1, 2:1, 1:2 ) were synthesized by desolvation method. The nanoparticles were modified with poly-L-lysine (PLL) for siRNA binding and decorated with hyaluronic acid (HA) to target laryngeal cancer cell line, Hep-2. HA/PLL/Alb-Ser NPs were individually loaded with siRNAs for casein kinase 2 (CK2), Absent, Small, or Homeotic-Like (ASH2L), and Cyclin D1 genes, which are overexpressed in Hep-2 cells. Downregulation of genes was confirmed by real-time PCR (RT-PCR). Size, morphological, and thermogravimetric characterizations revealed that Alb-Ser NPs having 2:1 () ratio are the most optimized formulation. Between 36.8 and 61.3% of siRNA entrapment efficiencies were achieved. HA/PLL-siRNA/Alb-Ser (2:1) NPs-mediated gene silencing resulted in a significant inhibition of cell growth and induction of apoptosis in cells. Our findings showed that HA/PLL/Alb-Ser (2:1) NPs were promising as a siRNA carrier. |
| Anahtar Kelimeler |
| Albumin | sericin | nanocarrier | siRNA | laryngeal cancer treatment |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Preparative Biochemistry and Biotechnology |
| Dergi ISSN | 1082-6068 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2019 |
| Cilt No | 49 |
| Sayı | 7 |
| Sayfalar | 1 / 12 |
| Doi Numarası | 10.1080/10826068.2019.1599395 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.1080/10826068.2019.1599395 |
| Atıf Sayıları | |
| WoS | 39 |
| Google Scholar | 48 |
