| Yazarlar (6) |
Hüseyin Gencay Keçeli
Hacettepe Üniversitesi, Türkiye |
|
Cem Bayram
Hacettepe Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Ekin ÇELİK
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Nuray Ercan
Türkiye |
|
Murat Demirbilek
Ankara Hacı Bayram Veli Üniversitesi, Türkiye |
|
Rahime Meral Nohutcu
Türkiye |
| Özet |
| To test the surface properties and in vitro effects of a new sequential release system on MC3T3-E1 cells for improved osseointegration. BMP6-loaded anodized titanium coated with PDGF containing silk fibroin (SF) may improve osseointegration. Titanium surfaces were electrochemically anodized, and SF layer was covered via electrospinning. Five experimental groups (unanodized Ti (Ti), anodized Ti (AnTi), anodized + BMP6-loaded Ti (AnTi-BMP6), anodized + BMP6 loaded + silk fibroin-coated Ti (AnTi-BMP6-SF), and anodized + BMP6-loaded + silk fibroin with PDGF-coated Ti (AnTi-BMP6-PDGF-SF)) were tested. After SEM characterization, contact angle analysis, and FTIR analysis, the amount of released PDGF and BMP6 was detected using ELISA. Cell proliferation (XTT), mineralization, and gene expression (RUNX2 and ALPL) were also evaluated. After successful anodization and loading of PDGF and BMP6, contact angle measurements showed hydrophobicity for TiO and hydrophilicity for protein-adsorbed surfaces. In FTIR, protein-containing surfaces exhibited amide-I, amide-II, and amide-III bands at 1600 cm -1700 cm , 1520 cm -1540 cm , and 1220 cm -1300 cm spectrum levels with a significant peak in BMP6- and/or SF-loaded groups at 1100 cm . PDGF release and BMP6 release were delayed, and relatively slower release was detected in SF-coated surfaces. Higher MC3T3-E1 proliferation and mineralization and lower gene expression of RUNX2 and ALPL were detected in AnTi-BMP6-PDGF-SF toward day 28. The new system revealed a high potential for an improved early osseointegration period by means of a better factor release curve and contribution to the osteoblastic cell proliferation, mineralization, and associated gene expression. |
| Anahtar Kelimeler |
| bone morphogenetic factor-6 | bone regeneration | dental implant | nanotechnology | nanotubes | osseointegration | osteoblast | platelet-derived growth factor | sequential release | silk fibroin |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Journal of Periodontal Research |
| Dergi ISSN | 0022-3484 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 08-2020 |
| Cilt No | 55 |
| Sayı | 5 |
| Sayfalar | 694 / 704 |
| Doi Numarası | 10.1111/jre.12756 |
| Makale Linki | http://dx.doi.org/10.1111/jre.12756 |
| Atıf Sayıları | |
| WoS | 18 |
| Google Scholar | 27 |
