| Yazarlar (3) |
Dr. Öğr. Üyesi Ekin ÇELİK
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Cem Bayram
Hacettepe Üniversitesi, Türkiye |
|
Emir Baki Denkbaş
Başkent Üniversitesi, Türkiye |
| Özet |
| Degenerative cartilage is the pathology of severe depletion of extracellular matrix components in articular cartilage. In diseases like osteoarthritis, misregulation of microRNAs contributes the pathology and collectively leads to disruption of the homeostasis. In this study chondroitin sulfate/hyaluronic acid/chitosan nanoparticles were prepared and successfully characterized chemically and morphologically. Results demonstrated higher chondroitin sulfate amounts led smaller nanoparticles, but lower surface zeta potential due to high electronegativity. After optimization of chondroitin sulfate amounts regarding size and charge, nanoparticles were loaded with microRNA-149-5p, a therapeutic miRNA downregulated in osteoarthritis, and evaluated focusing on their loading efficiency, release behaviour, cytotoxicity and gene transfection efficiency in vitro. Results showed all nanoparticle formulations were non-toxic and promising gene delivery agents, due to increased levels of microRNA-149-5p and decreased mRNA levels of microRNA's target, FUT-1. Highest gene transfection efficiency was obtained with the nanoparticle formulation which had the highest chondroitin sulfate load and smallest size. In addition, owing to their high chondroitin sulfate cargo, all nanoparticles were reported to enhance chondrogenesis, which was demonstrated by gene expression analysis and sulfated glycosaminoglycan (sGAG) staining. The obtained data suggest that the delivery of microRNA-149-5p via polysaccharide based carriers could achieve collaborative impact in cartilage regeneration and have a potential to enhance osteoarthritis treatment. |
| Anahtar Kelimeler |
| Hyaluronic acid | Chitosan | Chondroitin sulfate | Micro RNA | Nanoparticle | Chondrogenesis |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Materials Science & Engineering C-Materials for Biological Applications |
| Dergi ISSN | 0928-4931 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 09-2019 |
| Cilt No | 102 |
| Sayfalar | 756 / 763 |
| Doi Numarası | 10.1016/j.msec.2019.05.006 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S0928493119304485 |
| Atıf Sayıları | |
| WoS | 16 |
| Google Scholar | 19 |
