Synthesis of doxorubin loaded magnetic chitosan nanoparticles
Yazarlar (5)
Gozde Unsoy
R Khodahollah
Prof. Dr. Serap YALÇIN AZARKAN Türkiye
Pelin Mutlu
Ufuk Gunduz Kırşehir Ahi Evran Üniversitesi
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | J Biotechnol | ||
| Makale Dili | – | Basım Tarihi | 01-2014 |
| Cilt / Sayı / Sayfa | 1 / 4 / 10–21 | DOI | – |
| Makale Linki | https://scholar.google.com/scholar?cluster=832962981546629432&hl=en&oi=scholarr | ||
| UAK Araştırma Alanları |
Biyokimya & Moleküler Biyoloji
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| Özet |
| 27 Targeted drug delivery is a promising alternative to overcome the limitations of classical chemotherapy. In 28 an ideal targeted drug delivery system carrier nanoparticles would be directed to the tumor tissue and 29 selectively release therapeutic molecules. As a novel approach, chitosan coated magnetic nanoparticles 30 (CS MNPs) maintain a pH dependent drug delivery which provides targeting of drugs to the tumor site 31 under a magnetic field. Among various materials, chitosan has a great importance as a pH sensitive, 32 natural, biodegradable, biocompatible and bioadhesive polymer. The aim of this study was to obtain an 33 effective targeted delivery system for Doxorubicin, using chitosan coated MNPs. Different sized CS MNPs 34 were produced by in situ synthesis method. The anti-cancer agent Doxorubicin was loaded onto CS MNPs 35 which were characterized previously. Doxorubicin loading was confirmed by FTIR. Drug loading and 36 release characteristics, and stability of the nanoparticles were investigated. Our results showed that the 37 CS MNPs have pH responsive release characteristics. The cellular internalization of Doxorubicin loaded 38 CS MNPs were visualized by fluorescent microscopy. Doxorubicin loaded CS MNPs are efficiently taken 39 up by MCF-7 (MCF-7/S) and Doxorubicin resistant MCF-7 (MCF-7/1 μM) breast cancer cells, which 40 increases the efficacy of drug and also maintains overcoming the resistance of Doxorubicin in MCF-7/41 Dox cells. Consequently, CS MNPs synthesized at various sizes can be effectively used for the pH dependent 42 release of Doxorubicin in cancer cells. Results of this … |
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