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Phylogenetic analysis of black queen cell virusand deformed wing virus in honeybee coloniesinfected by mites in Van, Eastern Turkey       
Yazarlar
Zeynep Karapınar
Balıkesir Üniversitesi, Türkiye
Bekir Oğuz
Van Yüzüncü Yıl Üniversitesi, Türkiye
Ender Dinçer
Mersin Üniversitesi, Türkiye
Dr. Öğr. Üyesi Cihat ÖZTÜRK Dr. Öğr. Üyesi Cihat ÖZTÜRK
Van Yüzüncü Yıl Üniversitesi, Türkiye
Özet
This study aimed to determine the presence and prevalence of viral and parasitic infections causing high rates of colony loss in honey bee colonies in Van province, eastern Turkey. Twenty-six different apiaries were collected from five counties in Van province. These samples were tested by Reverse-Transcriptase PCR (RT-PCR) for acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), black queen cell virus (BQCV) and deformed wing virus (DWV). Selected positives were sequenced, phylogenetically analyzed and investigated in terms of Varroa. DWV and BQCV were identified in 69.23% (18/26) and 88.46% (23/26) of the bees respectively whereas ABPV and CBPV were not detected in the sampled apiaries. Results of the phylogenetic analysis of DWV and BQCV sequences showed 94-100% similarity to DWV and BQCV isolates obtained from Genbank. Prevalence of varroasis was 89% (23/26) in Van. The obtained samples were identified as Varroa destructor by morphological investigation. The study showed that viral and parasitic agents commonly infect honeybees in Van province, with high prevalence rates for BQCV and DWV. There was also a high degree of conservation of DWV and BQCV sequences distinct from DWV and BQCV isolates from other geographical regions. These findings, including current prevalence and phylogenetic analysis data for DWV, BQCV and varroazis in honeybees, are useful for future studies.
Anahtar Kelimeler
BQCV | DWV | Honey bee | PCR | Varroasis
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
Dergi ISSN 0025-8628
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 07-2018
Cilt No 74
Sayı 7
Sayfalar 460 / 465
Doi Numarası 10.21521/mw.5990