Controlling GRF4-GIF1 expression for efficient, genotype-independent transformation across wheat cultivars
Yazarlar (9)
Sadiye Hayta
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Mark A. Smedley
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Doç. Dr. Meltem BAYRAKTAR Kırşehir Ahi Evran Üniversitesi, Türkiye
Macarena Forner
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Anna Backhaus Norwich Research Park, İngiltere
Clare Lister
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Martha Clarke
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Cristobal Uauy
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
Simon Griffiths
Norwich Research Park, İngiltere
Norwich Research Park, İngiltere
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Plant Journal (Q1) | ||
| Dergi ISSN | 0960-7412 Dergi Bilgileri (2026) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2026 |
| Cilt / Sayı / Sayfa | 125 / 6 / 1–13 | DOI | 10.1111/tpj.70799 |
| Makale Linki | https://doi.org/10.1111/tpj.70799 | ||
| UAK Araştırma Alanları |
Bitkisel Biyoteknoloji
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| Özet |
| Wheat is a staple crop critical for global food security, and its continuous genetic improvement is essential to meet the demands of a growing population. Efficient, genotype‐independent transformation is a major bottleneck in wheat functional genomics and gene editing. The growth regulating factor (GRF)–GRF‐interacting factor (GIF) fusion technology enhances regeneration efficiency and broadens the range of transformable cultivars, but constitutive expression can reduce fertility and spikelet number. Here, we present an optimised Agrobacterium‐mediated wheat transformation protocol incorporating GRF4‐GIF1, tested across multiple tetraploid and hexaploid cultivars. Transformation efficiency was improved through adjustments in selection pressure, zeatin concentration, and promoter choice, with GRF4‐GIF1 consistently enabling successful transformation across genotypes. Tissue‐specific promoters and … |
| Anahtar Kelimeler |
| Agrobacterium | crop transformation | gene editing | genotype independence | GRF4-GIF | wheat |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 1 |
| Scopus | 2 |
| Google Scholar | 4 |