Ancient DNA (aDNA) extraction and amplification from 3500-year-old charred economic crop seeds from Kaymakçı in Western Turkey: comparative sequence analysis using the 26S rDNA gene
Yazarlar (6)
Asiye Ciftci
Middle East Technical University (Metu), Türkiye
Dr. Öğr. Üyesi Funda ÖZDEMİR DEĞİRMENCİ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Christina Luke
Koç University, Türkiye
Christopher H. Roosevelt
Koç University, Türkiye
John M. Marston
College Of Arts & Sciences, Amerika Birleşik Devletleri
Zeki Kaya
Middle East Technical University (Metu), Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Genetic Resources and Crop Evolution (Q2) | ||
| Dergi ISSN | 0925-9864 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2019 |
| Cilt / Sayı / Sayfa | 66 / 6 / 1279–1294 | DOI | 10.1007/s10722-019-00783-9 |
| Makale Linki | https://doi.org/10.1007/s10722-019-00783-9 | ||
| Özet |
| Ancient DNA (aDNA) from 3500–4000 years old seeds of Triticum aestivum L. or Triticum durum Dest., Vicia ervillia (L) Willd., Cicer arietinum L. and Vitis vinifera L. excavated from the archaeological site of Kaymakçı was successfully extracted using various isolation methods. The genomic DNA of each species was amplified with respect to the 26S ribosomal DNA (rDNA) gene further using the aDNA of the seeds. The reasons for successful DNA extraction and amplification are likely due to (1) preservation of certain ancient seed specimens in good conditions and (2) use of improved DNA extraction and amplification methods. The results indicate that all seeds were identified correctly by the DNA sequence data from the 26S rDNA gene. Specifically, a morphologically unidentified wheat seed from Kaymakçı was characterized by DNA sequence data as bread wheat (Triticum aestivum). Comparative sequence … |
| Anahtar Kelimeler |
| 26S rDNA gene | Ancient DNA | Comparative sequence analysis | Domestication | Genomic DNA | Kaymakçı |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 6 |
| SCOPUS | 6 |
| Google Scholar | 13 |