Therapeutic effects of vitamin D and IL-22 on methotrexate-induced mucositis in mice
Yazarlar (13)
Ebru Yılmaz Erciyes Üniversitesi, Türkiye
Zehra Büşra Azizoğlu
Kübra Aslan Türkiye
Dr. Öğr. Üyesi Şerife ERDEM Türkiye
Yeşim Haliloğlu
Pınar Alişan Suna
Arzu Hanım Yay Türkiye
Kemal Deniz Türkiye
Abdulkadir Taşdemir Türkiye
Sedat Per Türkiye
Ekrem Ünal Türkiye
Musa Karakükcü Türkiye
Türkan Patıroğlu
Türkiye
Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Anti-Cancer Drugs (Q4) | ||
| Dergi ISSN | 1473-5741 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2022 |
| Cilt / Sayı / Sayfa | 33 / 1 / 11–18 | DOI | 10.1097/CAD.0000000000001128 |
| Makale Linki | https://journals.lww.com/anti-cancerdrugs/Abstract/9000/Therapeutic_effects_of_vitamin_D_and_IL_22_on.98349.aspx | ||
| Özet |
| Mucositis is a common side effect of cancer therapies and transplant conditioning regimens. Management of mucositis involves multiple approaches from oral hygiene, anti-inflammatory, anti-apoptotic, cytoprotective, and antioxidant agents, to cryo-therapy, physical therapy, and growth factors. There is room for novel, affordable treatment options, or improvement of currently available therapies. Vitamin D has been shown to regulate mucosa-resident cell populations such as Th17 or innate lymphoid cells and critical mucosal cytokine IL-22; however, their therapeutic potential has not been put to test in preclinical mouse models. In this study, we aimed to test the therapeutic potential of vitamin D injections and IL-22 overexpression in a murine model of chemotherapy-induced mucositis. Balb/c mice were given daily intraperitoneal injections of vitamin D. Mucositis was induced by methotrexate. Another group received IL-22 plasmid via hydrodynamic gene delivery. Weight loss and intestinal histopathology, intestinal levels of cytokines IL-22, IL-17A, GM-CSF, IL-23, IFN-γ, TNF-α, and IL-10, and number of intestinal lamina propria B cell, neutrophil, and total innate lymphoid cells were quantified. Daily vitamin D injections ameliorated intestinal inflammation and elevated intestinal IL-22 levels compared with control groups. Temporal overexpression of IL-22 by hydrodynamic gene delivery slightly increased intestinal IL-22 but failed to confer significant protection from mucositis. To our knowledge, this is the first experimental demonstration in an animal model of mucositis of therapeutic use of vitamin D and IL-22 supplementation and our results with vitamin D suggest it may have merit in further trials in human mucositis patients. |
| Anahtar Kelimeler |
| innate lymphoid cell | IL-22 | methotrexate | mucositis | vitamin D |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 7 |