| Yazarlar (4) |
Besim Cam
Kirsehir Ahi Evran University, Türkiye |
Dr. Öğr. Üyesi Lokman HİZMALİ
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Elif Sevim
Kirsehir Ahi Evran University, Türkiye |
|
Rukiye Akyol
Teaching & Res Hosp, Türkiye |
| Özet |
| Ventilator-associated pneumonia (VAP) is the most common infection encountered in intensive care units and is closely linked with elevated mortality, morbidity, and healthcare expenditure. The predominant pathogens responsible for VAP are multidrug-resistant (MDR) Gram-negative bacteria, including Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli. This study aimed to investigate the molecular antibiotic resistance profiles of bacterial isolates from hospital-acquired VAP cases. Conducted between 30 November 2022 and 30 November 2023 at Kırşehir Training and Research Hospital, resistance genes were identified using Polymerase Chain Reaction (PCR), while clonal relatedness and genotyping were assessed through Repetitive Extragenic Palindromic-PCR (rep-PCR) and Multi-Locus Sequence Typing (MLST). The most frequently isolated organisms were A. baumannii (46.2%), K. pneumoniae (42.3%), and P. aeruginosa (7.7%). A. baumannii strains exhibited 100% resistance to ciprofloxacin and carbapenems, and 70.83% to colistin. K. pneumoniae strains demonstrated 94.73% resistance to carbapenems and 100% to piperacillin-tazobactam and colistin. Molecular analyses identified bla, bla, bla, bla, bla, and bla in A. baumannii, and bla, bla, bla, bla, bla, and bla in K. pneumoniae. Nine isolates (17%) were identified as transconjugants. MLST analysis revealed K. pneumoniae ST2096 and A. baumannii ST2 as predominant. Clones not previously reported in Türkiye A. baumannii ST78 and K. pneumoniae ST45, ST437, and ST1128 were also detected. This study provides a comprehensive molecular characterisation of VAP pathogens based on an extensive dataset. The results underscore the need for stricter infection control, restrained use of broad-spectrum antibiotics, and ongoing surveillance of resistance mechanisms. |
| Anahtar Kelimeler |
| Ventilator-associated pneumonia | Antimicrobial resistance | Molecular genotyping | Acinetobacter baumannii | Klebsiella pneumoniae |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | FOLIA MICROBIOLOGICA |
| Dergi ISSN | 0015-5632 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2025 |
| DOI Numarası | 10.1007/s12223-025-01392-z |
| Atıf Sayıları |
