| Yazarlar (4) |
Dr. Öğr. Üyesi Sevil KESTANE
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Alican Bilden
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Merve Kahraman
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Muttalip Cicek
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Medical leech therapy has been the subject of many scientific studies for years. However, the material basis of the leech in cell growth was not yet clear. First, this study proposes to evaluate the bioactivity of leech saliva extract (LSE) in mesenchymal stem cell culture. Effect of LSE was investigated on the viability and proliferation of bone marrow-derived mesenchymal stem cells in vitro. BM-MSCs were cultured and treated with 2 different doses of LSE. The proliferation rates and viability percentages of cells were calculated during 3 passages. The experiments were performed in three groups: BM-MSCs cultured with a high (1.00 mL) dose of LSE-Dose1 (LSE-D1); MSCs cultured with a low (0.2 mL) dose of LSE-Dose2 (LSE-D2); and MSC culture groups. LSE increased cell proliferation and viability in vitro (P< 0.05). LSE-D2 showed the highest activity, increased cell proliferation, and cell viability in vitro (P< 0.05). Cell culture can affect the cell population profile and change cell differentiation. These findings demonstrated that LSE-D2 interferes with MSCs' growth kinetics. Leech salvia extracts increased cell proliferation and viability in stem cell culture. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Indian Journal of Experimental Biology (IJEB) |
| Dergi ISSN | 0019-5189 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | |
| Dergi Grubu | Q4 |
| Makale Dili | İngilizce |
| Basım Tarihi | 10-2025 |
| Cilt No | 63 |
| Sayı | 7 |
| Sayfalar | 605 / 611 |
