| Yazarlar (9) |
Mustafa Numan Bucak
Selçuk Üniversitesi, Türkiye |
|
Morteza Taravat
Urmia University Of Medical Sciences, İran |
|
Oya Korkmaz
Malatya Turgut Ozal University, Türkiye |
|
Ali Soliemanzadeh
Urmia University Faculty Of Veterinary Medicine, İran |
|
Mojtaba Karimpour
Urmia University Of Medical Sciences, İran |
|
Mohammad Saber Maroufi
Urmia University Of Medical Sciences, İran |
|
Neda Abedpour
Urmia University Of Medical Sciences, İran |
Dr. Öğr. Üyesi Sadık KÜÇÜKGÜNAY
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Tohid Rezaei Topraggaleh
Urmia University Of Medical Sciences, İran |
| Özet |
| Glycerol is commonly used as a cryoprotectant in bovine sperm cryopreservation; however, its cytotoxic effects can negatively impact sperm quality. This study aimed to reduce glycerol concentration in semen extenders by supplementing with natural antioxidants (taxifolin and fetuin) and the disaccharide trehalose. Twelve ejaculates were collected from three bulls and initially evaluated, then diluted in a tris-egg yolk (TEY) extender containing either 7 % or 4 % glycerol. The control group received 7 % glycerol (TEY), while experimental groups included G7F (TEY + fetuin), G7T (TEY + taxifolin), G7M (TEY + fetuin + taxifolin), and 4 % glycerol groups supplemented with 50 mM trehalose: G4 (TEY + trehalose), G4F (TEY + fetuin), G4T (TEY + taxifolin), and G4M (TEY + fetuin + taxifolin). Post-thaw evaluations included assessments of sperm motility, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), DNA fragmentation, and oxidative stress markers. The G4F and G4T groups exhibited significantly higher total and progressive motility, viability, plasma membrane integrity, and MMP compared to other groups (p < 0.05). Furthermore, these groups showed significantly lower malondialdehyde (MDA) levels, indicating reduced lipid peroxidation and oxidative stress (p < 0.05). In conclusion, co-supplementation of 50 mM trehalose with 50 μM taxifolin and 5 mg/mL fetuin in a cryopreservation extender containing 4 % glycerol may enhance the post-thaw quality of bull spermatozoa. |
| Anahtar Kelimeler |
| Bull sperm | Cryopreservation | Fetuin | Glycerol | Taxifolin | Trehalose |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Cryobiology |
| Dergi ISSN | 0011-2240 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2025 |
| Cilt No | 121 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.cryobiol.2025.105330 |
| Atıf Sayıları |
