Somatic Embryogenesis and Plant Regeneration From ımmature ınflorescences of Hyparrhenia hirta (L.) Stapf
Yazarlar (4)
R Hatipoğlu
Cu Hesemann
A Gland
T Tükel
| Bildiri Türü | Tebliğ/Bildiri | Bildiri Dili | İngilizce |
| Bildiri Alt Türü | Tam Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) | ||
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum | ||
| Kongre Adı | XVıı. ınternational Grassland Congress | ||
| Kongre Tarihi | 13-02-1993 / 16-02-1993 | ||
| Basıldığı Ülke | Yeni Zelanda | Basıldığı Şehir | |
| Bildiri Linki | https://uknowledge.uky.edu/igc/1993/session27/9/ | ||
| UAK Araştırma Alanları |
Çayır-Mera ve Yem Bitkileri
|
||
| Özet |
| The study was conducted to determine an efficient system for in vitro culture and Hyparrhenia hirta, The effects of 2 different auxins (2, 4-D and dicamba) at 3 concentrations (4.5 puM, 226 uM and 45, 2 pih) and 2 ecotypes with different chromosome numbers (2n= 36 and 2n= 54) on callus induction and plantlet regeneration from immature inflorescences of Hyparrhenia hirta were investigated. Inflorescence segments of 5 mm length from greenhouse-grown plants were cultured on a modified Linsmaier and Skoog medium supplemented with 30 g/l agar, Large differences in frequency of callus induction and callus weight were observed among the different ecotypes of Hyparrhenia hirta, but the regeneration rate was not influenced by ecotypes, Callus induction and callus weight were not affected by the ecotype chromosome numbers. The auxins tested were not statistically different in the effectiveness for the callus induction, Dicamba produced higher mean callus weights than 2, 4-D, In contrast, 2, 4-D was more effective than dicamba for plant regeneration, and the highest averaged regeneration rate was obtained from the explants cultured on medium with 226 piłM 2, 4-D. |
| Anahtar Kelimeler |
