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Assessment of genetic diversity in walnut (Juglans regia L.) genotypes from southern and southeastern Kazakhstan using microsatellite markers       
Yazarlar (9)
Makpal Nurzhuma
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Alma Kokhmetova
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Madina Kumarbayeva
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Zhenis Keishilov
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Kanat Bakhytuly
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Ardak Bolatbekova
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Assiya Kokhmetova
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Kanat Mukhametzhanov
Institute Of Plant Biology And Biotechnology, Almaty, Kazakistan
Doç. Dr. Kadir AKAN Doç. Dr. Kadir AKAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
The walnut (Juglans regia L.) holds a significant position among Kazakhstan’s nut crops, both in terms of cultivation and consumption. Although genetic resources of walnut are accessible within the country, they remain underexplored, yet they represent a valuable foundation for breeding initiatives and the development of locally adapted varieties. Currently, a comprehensive genetic profile of these resources is lacking, which hampers their effective utilization. The aim of this research was to evaluate the genetic diversity within a collection of walnut accessions collected from the southern and southeastern regions of Kazakhstan, including Almaty and Turkestan regions. To achieve this, eight SSR molecular markers were employed, providing insights into the genetic structure and relationships among the germplasm. All markers exhibited a high degree of polymorphism, with the WGA276 locus standing out as the most informative, displaying the greatest number of alleles and a high Shannon diversity index. The average expected heterozygosity (He) was 0.704 and was significantly higher than the observed heterozygosity (Ho = 0.547), which was confirmed by t-test (t = −6.426, p < 0.05). The findings indicated substantial genetic variation and intra-population polymorphism: on average, 5.875 alleles per locus were identified, and the Shannon index was 1.444. The population from Turkestan region (population 1, Sairam-Ugam) demonstrated the highest levels of diversity. Analyses of genetic structure, conducted through STRUCTURE, PCoA, and UPGMA methods, confirmed the existence of two genetically distinct groups exhibiting considerable diversity. Future research should focus on conserving the highly diverse populations in the Turkestan region to facilitate the development of stress-tolerant varieties. These findings highlight the importance of conserving and harnessing the genetic resources of Kazakhstan’s J. regia populations for future breeding efforts.
Anahtar Kelimeler
breeding | genetic diversity | microsatellite markers | polymorphism | population structure | walnut
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale
Dergi Adı Horticulturae
Dergi ISSN 2311-7524 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Dergi Grubu Q1
Makale Dili İngilizce
Basım Tarihi 07-2025
Cilt No 11
Sayı 7
Sayfalar 1 / 16
Doi Numarası 10.3390/horticulturae11070810
Makale Linki https://www.mdpi.com/2311-7524/11/7/810