| Yazarlar |
Mustafa Nazıroğlu
Süleyman Demirel Üniversitesi, Türkiye |
|
Cemil Özgül
|
Doç. Dr. Bilal ÇİĞ
Süleyman Demirel Üniversitesi, Türkiye |
|
Salih Doğan
|
|
Abdülhadi Cihangir Uğuz
Süleyman Demirel Üniversitesi, Türkiye |
| Özet |
| Glutathione (GSH) is the most abundant thiol antioxidant in mammalian cells and maintains thiol redox in the cells. GSH depletion has been implicated in the neurobiology of sensory neurons. Because the mechanisms that lead to melastatin-like transient receptor potential 2 (TRPM2) channel activation/inhibition in response to glutathione depletion and 2-aminoethyldiphenyl borinate (2-APB) administration are not understood, we tested the effects of 2-APB and GSH on oxidative stress and buthionine sulfoximine (BSO)-induced TRPM2 cation channel currents in dorsal root ganglion (DRG) neurons of rats. DRG neurons were freshly isolated from rats and the neurons were incubated for 24 h with BSO. In whole-cell patch clamp experiments, TRPM2 currents in the rat were consistently induced by H2O2 or BSO. TRPM2 channels current densities and cytosolic free Ca2+ content of the neurons were higher in BSO and H2O2 groups than in control. However, the current densities and cytosolic Ca2+ release were also higher in the BSO + H2O2 group than in the H2O2 alone. When intracellular GSH is introduced by pipette TRPM2 channel currents were not activated by BSO, H2O2 or rotenone. BSO and H2O2-induced Ca2+ gates were blocked by the 2-APB. Glutathione peroxidase activity, lipid peroxidation and GSH levels in the DRG neurons were also modulated by GSH and 2-APB inhibition. In conclusion, we observed the protective role of 2-APB and GSH on Ca2+ influx through a TRPM2 channel in intracellular GSH depleted DRG neurons. Since cytosolic glutathione depletion is a common feature of neuropathic pain and diseases of sensory neuron, our findings are relevant to the etiology of neuropathology in DRG neurons. |
| Anahtar Kelimeler |
| Buthionine sulfoximine | Glutathione | Oxidative stress | Sensory neurons | TRPM2 channel antagonist |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF MEMBRANE BIOLOGY |
| Dergi ISSN | 0022-2631 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 08-2011 |
| Cilt No | 242 |
| Sayı | 3 |
| Sayfalar | 109 / 118 |
| Doi Numarası | 10.1007/s00232-011-9382-6 |
| Atıf Sayıları | |
| WoS | 54 |
| SCOPUS | 60 |
