Modulation of oxidative stress and Ca2 mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum

Naziroǧlu, M.; ÇİĞ, BİLAL; Özgül, C.

doi:10.1016/j.neuroscience.2014.01.006

Modulation of oxidative stress and Ca2 mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum

Yazarlar (3)

M. Naziroǧlu
Süleyman Demirel Üniversitesi, Türkiye

Doç. Dr. Bilal ÇİĞ Süleyman Demirel University, Faculty Of Medicine, Türkiye

C. Özgül İstanbul Medipol Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Neuroscience
Dergi ISSN	0306-4522 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	İngilizce	Basım Tarihi	03-2014
Cilt / Sayı / Sayfa	263 / 1 / 27–35	DOI	10.1016/j.neuroscience.2014.01.006
Makale Linki	http://linkinghub.elsevier.com/retrieve/pii/S0306452214000104

Özet

A main component of St. John's Wort (Hypericum perforatum, HP) is hyperforin which has antioxidant properties in dorsal root ganglion (DRG) neurons, due to its ability to modulate NADPH oxidase and protein kinase C. Recent reports indicate that oxidative stress through NADPH oxidase activates TRPM2 channels. HP may be a useful treatment for Ca²⁺ entry and oxidative stress through modulation of TRPM2 channels in the DRG. We aimed to investigate the protective role of HP on Ca²⁺ entry and oxidative stress through TRPM2 channels in DRG neurons of rats.The native rat DRG neurons were used in whole-cell patch-clamp, Fura-2 and antioxidant experiments. Appropriate, nontoxic concentrations and incubation times for HP were determined in the DRG neurons by assessing cell viability. The H2O2-induced TRPM2 currents were inhibited by 2-aminoethyl diphenylborinate (2-APB) and N-(p-amylcinnamoyl)anthranilic acid (ACA). TRPM2 current densities and cytosolic free Ca²⁺ concentration in the neurons were also reduced by HP (2 and 24h). In Fura-2 experiments, cytosolic Ca²⁺ mobilization was reduced by voltage-gated calcium channel blockers (verapamil+diltiazem, V+D) and HP. Glutathione peroxidase activity and GSH values in the DRG were high in HP, 2-APB and V+D groups although lipid peroxidation level was low in the groups.In conclusion, we observed a protective role for HP on Ca²⁺ entry through a TRPM2 channel in the DRG neurons. Since over-production of oxidative stress and Ca²⁺ entry are implicated in the pathophysiology of neuropathic pain and neuronal inflammation, our findings may be relevant to the etiology and treatment of neuropathology in DRG neurons. © 2014 IBRO.

Anahtar Kelimeler

Science Direct

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
WoS	30
SCOPUS	34

Modulation of oxidative stress and Ca2 mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum

Dergi Adı	NEUROSCIENCE
Yayıncı	Elsevier Ltd
Açık Erişim	Hayır
ISSN	0306-4522
E-ISSN	1873-7544
CiteScore	5,6
SJR	1,008
SNIP	0,757

Modulation of oxidative stress and Ca2 mobilization through TRPM2 channels in rat dorsal root ganglion neuron by Hypericum perforatum

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