| Yazarlar (5) |
Esin Sakallı Çetin
Muğla Sıtkı Koçman Üniversitesi, Türkiye |
|
Mustafa Nazıroğlu
Süleyman Demirel Üniversitesi, Türkiye |
Doç. Dr. Bilal ÇİĞ
Süleyman Demirel Üniversitesi, Türkiye |
|
İshak Suat Övey
Süleyman Demirel University, Faculty Of Medicine, Türkiye |
|
Pınar Aslan Koşar
Süleyman Demirel Üniversitesi, Türkiye |
| Özet |
| Background: In breast cancers, calcium signaling is a main cause of proliferation and apoptosis of breast cancer cells. Although previous studies have implicated the transient receptor potential vanilloid 1 (TRPV1) cation channel, the synergistic inhibition effects of selenium (Se) and cisplatin in cancer and the suppression of ongoing apoptosis have not yet been investigated in MCF-7 breast cancer cells. This study investigates the anticancer properties of Se through TRPV1 channel activity in MCF-7 breast cancer cell line cultures when given alone or in combination with cisplatin. Materials: The MCF-7 cells were divided into four groups: the control group, the Se-treated group (200nM), the cisplatin-treated group (40M) and the Se+cisplatin-treated group. Results: The intracellular free calcium ion concentration and current densities increased with TRPV1 channel activator capsaicin (0.01mM), but they decreased with the TRPV1 blocker capsazepine (0.1mM), Se, cisplatin, and Se+cisplatin incubations. However, mitochondrial membrane depolarization, apoptosis, and the caspase 3, and caspase 9 values increased in the Se-treated group and the cisplatin-treated group, although Western blot (procaspase 3 and 9) results and the cell viability levels decreased with the Se and Se+cisplatin treatments. Apoptosis and caspase-3 were further increased with the Se+cisplatin treatment. Intracellular reactive oxygen species production increased with the cisplatin treatment, but not with the Se treatment. Conclusion: This study's results report, for the first time, that at a cellular level, Se and cisplatin interact on the same intracellular toxic cascade, and the combination of these two drugs can result in a remarkable anticancer effect through modulation of the TRPV1. |
| Anahtar Kelimeler |
| Apoptosis | breast cancer | calcium signaling | cisplatin | oxidative stress | TRPV1 channel |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION |
| Dergi ISSN | 1079-9893 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2017 |
| Cilt No | 37 |
| Sayı | 1 |
| Sayfalar | 84 / 93 |
| Doi Numarası | 10.3109/10799893.2016.1160931 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.3109/10799893.2016.1160931 |
| Atıf Sayıları | |
| WoS | 63 |
| SCOPUS | 67 |
