| Yazarlar |
Mustafa Nazıroğlu
Süleyman Demirel Üniversitesi, Türkiye |
|
Walter Blum
|
|
Katalin Josvay
|
Doç. Dr. Bilal ÇİĞ
Süleyman Demirel Üniversitesi, Türkiye |
|
Thomas Henzi
|
|
Zoltan Olah
|
|
Csaba Vizler
|
|
Beat Schwaller
|
|
Laszlo Pecze
|
| Özet |
| Menthol is a naturally occurring monoterpene alcohol possessing remarkable biological properties including antipruritic, analgesic, antiseptic, anti-inflammatory and cooling effects. Here, we examined the menthol-evoked Ca2+ signals in breast and prostate cancer cell lines. The effect of menthol (50-500 mu M) was predicted to be mediated by the transient receptor potential ion channel melastatin subtype 8 (TRPM8). However, the intensity of menthol-evoked Ca2+ signals did not correlate with the expression levels of TRPM8 in breast and prostate cancer cells indicating a TRPM8-independent signaling pathway. Menthol-evoked Ca2+ signals were analyzed in detail in Du 145 prostate cancer cells, as well as in CRISPR/Cas9 TRPM8-knockout Du 145 cells. Menthol (500 mu M) induced Ca2+ oscillations in both cell lines, thus independent of TRPM8, which were however dependent on the production of inositol trisphosphate. Results based on pharmacological tools point to an involvement of the purinergic pathway in menthol-evoked Ca2+ responses. Finally, menthol (50-500 mu M) decreased cell viability and induced oxidative stress independently of the presence of TRPM8 channels, despite that temperature-evoked TRPM8-mediated inward currents were significantly decreased in TRPM8-knockout Du 145 cells compared to wild type Du 145 cells. |
| Anahtar Kelimeler |
| Ca oscillations 2+ | Menthol | Oxidative stress | Purinergic signaling | TRPM8 |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | REDOX BIOLOGY |
| Dergi ISSN | 2213-2317 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2018 |
| Cilt No | 14 |
| Sayı | 1 |
| Sayfalar | 439 / 449 |
| Doi Numarası | 10.1016/j.redox.2017.10.009 |
| Makale Linki | https://linkinghub.elsevier.com/retrieve/pii/S2213231717307541 |
| Atıf Sayıları | |
| WoS | 27 |
| SCOPUS | 33 |
