Melatonin potentiates chemotherapy induced cytotoxicity and apoptosis in ratpancreatic tumor cells
Yazarlar (7)
Abdulhadi C. Uguz
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Doç. Dr. Bilal ÇİĞ
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Javier Espino
Universidad De Extremadura, İspanya
Ignacio Bejarano
Universidad De Extremadura, İspanya
Mustafa Naziroglu
Süleyman Demirel University, Faculty Of Medicine, Türkiye
Ana B. Rodríguez
Universidad De Extremadura, İspanya
José A. Pariente
Universidad De Extremadura, İspanya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Pineal Research | ||
| Dergi ISSN | 0742-3098 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2012 |
| Cilt / Sayı / Sayfa | 53 / 1 / 91–98 | DOI | 10.1111/j.1600-079X.2012.00974.x |
| Makale Linki | http://hdl.handle.net/10662/20499 | ||
| Özet |
| Melatonin has antitumor activity via several mechanisms including its antiproliferative and proapoptotic effects in addition to its potent antioxidant action. Thus, melatonin has proven useful in the treatment of tumors in association with chemotherapeutic drugs. This study was performed to evaluate the effect of melatonin on the cytotoxicity and apoptosis induced by three different chemotherapeutic agents, namely 5-fluorouracil (5-FU), cisplatin, and doxorubicin in the rat pancreatic tumor cell line AR42J. We found that both melatonin and the three chemotherapeutic drugs induce a time-dependent decrease in AR42J cell viability, reaching the highest cytotoxic effect after 48 hr of incubation. Furthermore, melatonin significantly augmented the cytotoxicity of the chemotherapeutic agents. Consistently, cotreatment of AR42J cells with each of the chemotherapeutic agents in the presence of melatonin increased the population of apoptotic cells, elevated mitochondrial membrane depolarization, and augmented intracellular reactive oxygen species (ROS) production compared to treatment with each chemotherapeutic agent alone. These results provide evidence that in vitro melatonin enhances chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor AR42J cells and, therefore, melatonin may be potentially applied to pancreatic tumor treatment as a powerful synergistic agent in combination with chemotherapeutic drugs. |
| Anahtar Kelimeler |
| Apoptosis | AR42J cells | Chemotherapy | Cytotoxicity | Melatonin |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| WoS | 155 |
| SCOPUS | 156 |