| Yazarlar (5) |
Dr. Öğr. Üyesi Alican BİLDEN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Arş. Gör. Merve KAHRAMAN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Nadia İbrahim Kamil Kamil
|
|
Gülşah Özkula
|
Prof. Dr. Muttalip ÇİÇEK
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Objective: The permanent preparation of helminths and arthropods is of great importance for both research and educational purposes. The simplicity and applicability of this process ensures long-term preservation of the preparations. Strong adhesives such as glycerin gel, entellan and Canada balsam are widely used to increase the quality and durability of permanent preparations. However, these traditional mounting media have various limitations such as yellowing over time, brittleness, moisture sensitivity and long hardening times. The aim of this study is to evaluate the quality, durability and effectiveness of the helminths and arthropods embedding technique in epoxy resin and to determine its advantages compared to traditional methods. Methods: In the laboratory environment, species such as leeches (Hirudo sp.), Fasciola sp., Taenia sp., Enterobius vermicularis; arthropods such as hard ticks (Ixodidae), Reduviidae, Anopheles sp. were made permanent using epoxy resin. The samples were first subjected to appropriate fixation and dehydration processes, then carefully positioned and embedded in epoxy resin. The applied method was optimized to preserve the morphological integrity and three-dimensional structure of the parasites. Results: Epoxy resin has offered significant advantages such as high optical transparency, superior mechanical strength, the ability to create three-dimensional preparations and the capacity to preserve fine morphological details of parasites for a long time. It was observed that the preparations prepared in the study remained stable for a long time without deteriorating their physical structures and losing their optical properties. Compared to traditional methods, epoxy resin was found to provide a structure that is more resistant to environmental factors and does not deform over time. Conclusion: Epoxy resin has been evaluated as a fast and practical technique that provides high mounting quality for both helminths and arthropods. Its ability to maintain optical clarity, mechanical durability, and structural integrity over time makes this method a promising alternative for long-term specimen preservation and microscopic examination. The use of epoxy resin for educational and research purposes offers a valuable contribution to parasitology laboratories. |
| Anahtar Kelimeler |
| Arthropods | Artropodlar | epoksi reçinesi | epoxy resin | helminths | helmintler | kalıcı preparat | montaj teknikleri | mounting techniques | permanent preparation |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Türk Hijyen ve Deneysel Biyoloji Dergisi |
| Dergi ISSN | 0377-9777 Scopus Dergi |
| Dergi Tarandığı Indeksler | TR DİZİN |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2025 |
| Cilt No | 82 |
| Sayı | 2 |
| Sayfalar | 303 / 310 |
| Doi Numarası | 10.5505/TurkHijyen.2025.32067 |
| Makale Linki | https://www.turkhijyen.org/ |
| Atıf Sayıları |
