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Rapid Cultivation of Acanthamoeba spp. Isolated From Environmental Samples Using Nanocomposite and Leech Saliva on Non-Nutrient Agar      
Yazarlar (6)
Dr. Öğr. Üyesi Alican BİLDEN Dr. Öğr. Üyesi Alican BİLDEN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Erdal Ertaş
Türkiye
Arş. Gör. Merve KAHRAMAN Arş. Gör. Merve KAHRAMAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Servet Tural
Dicle Üniversitesi, Türkiye
Bilsen Tural
Dicle Üniversitesi, Türkiye
Prof. Dr. Muttalip ÇİÇEK Prof. Dr. Muttalip ÇİÇEK
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
Objective: Acanthamoeba spp. are microscopic single-celled protozoa commonly found in the environment, particularly in soil, water sources, and dust. These parasites are associated with serious infections such as Acanthamoeba keratitis and granulomatous amebic encephalitis. Recent epidemiological studies have highlighted a significant increase in Acanthamoeba keratitis cases. Current treatment methods are generally effective only in the early stages and show limited success when applied late, emphasizing the urgent need for more effective therapeutic and cultivation approaches. The laboratory cultivation of Acanthamoeba spp. is traditionally performed using axenic or monoxenic cultures. However, these methods have notable drawbacks, including the loss of virulence, reduced encystment capability, errors in bacterial inoculation, and time-consuming procedures. To address these limitations, nanotechnological approaches have been proposed. Nanotechnology offers innovative solutions for developing new drug formulations and diagnosing, preventing, and treating various diseases. Methods: In this study, we developed humic acid-coated magnetic nanocomposites capable of adhering to the cyst wall of Acanthamoeba spp. without causing damage. Experimental results demonstrated that these nanocomposites bind with high affinity to macromolecules on the cyst wall, facilitating the aggregation of parasites in the pellet. Additionally, biologically enriched leech saliva was incorporated into the culture medium to enhance the growth rate. Leech saliva provides a rich source of organic matter and bioactive molecules that promote cell division. The addition of leech saliva resulted in a significant increase in the growth rate of Acanthamoeba spp., with maximum growth density observed at 120 h. Results: These findings indicate that humic acid-coated magnetic nanocomposites and leech saliva -enriched culture media offer a promising alternative to conventional methods for the rapid and efficient cultivation of Acanthamoeba spp. Our study concludes that humic acid-coated magnetic nanocomposites effectively concentrate parasites, increasing their quantitative density, while leech saliva provides a nutrient-rich environment that stimulates trophozoite feeding and division. Conclusion: This study is noteworthy for presenting an innovative and effective method for the rapid laboratory cultivation and potential treatment of Acanthamoeba spp.
Anahtar Kelimeler
Acanthamoebaspp | Hirudo verbana | Nanotechnology | Non-Nutrient Agar
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Acta Parasitologica
Dergi ISSN 1896-1851 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 01-2025
Cilt No 70
Sayı 104
Sayfalar 1 / 9
Doi Numarası 10.1007/s11686-025-01053-8
Makale Linki https://link.springer.com/article/10.1007/s11686-025-01053-8