Extracellular vesicles therapy alleviates cisplatin-ınduced testicular tissue toxicity in a rat model
Yazarlar (8)
Dr. Öğr. Üyesi Halime TOZAK YILDIZ Kırşehir Ahi Evran Üniversitesi, Türkiye
Dr. Öğr. Üyesi Kübra Tuğçe KALKAN Kırşehir Ahi Evran Üniversitesi, Türkiye
Numan Baydilli Erciyes University, Türkiye
Zeynep Burçin Gönen Erciyes University, Türkiye
Özge Cengiz Mat Erciyes University, Türkiye
Eda Okur Türkiye
Gözde Özge Önder Erciyes University, Türkiye
Arzu Hanım Yay Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | PLOS ONE (Q2) | ||
| Dergi ISSN | 1932-6203 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2025 |
| Cilt / Sayı / Sayfa | 20 / 5 / 1–18 | DOI | 10.1371/journal.pone.0314093 |
| Makale Linki | https://doi.org/10.1371/journal.pone.0314093 | ||
| Özet |
| Purpose Cisplatin is a commonly used chemotherapy agent effective against various cancers, however it induces significant gonadotoxicity and infertility due to its adverse effects on testicular function. The underlying mechanisms of cisplatin-induced testicular damage include oxidative stress and dysregulated autophagy. This study investigates the potential of extracellular vesicles (EVs) to mitigate cisplatin-induced testicular damage through their regenerative, antioxidant, and autophagy-modulating properties. Methods In the testicular toxicity model, thirty-two male rats were randomly divided into four groups (n = 8): control, EVs-only, Cis-only, and Cis + EVs. A single intraperitoneal dose of 7.5mg/kg cisplatin was administered on the first day. On the six day, the EVs treatment group received a single dose of EVs (8x107/100μl) intravenously. Animals were sacrificed on day eight. Testicular histoarchitecture was assessed via hematoxylin and eosin staining. Sperm parameters, including motility and count, were measured using light microscopy. Hormone levels (testosterone and inhibin) were determined via enzyme-linked immunosorbent assay (ELISA). Oxidative stress markers, such as glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), catalase (CAT), and is a metabolite malondialdehyde (MDA), were quantified using colorimetric assays. Autophagy and steroidogenesis were evaluated through immunohistochemical analysis of Beclin-1, p62, LC3–2, SF-1, and StAR. Results Cisplatin exposure caused significant testicular damage, characterized by reduced germinal epithelium and degeneration of seminiferous tubules (p < … |
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BM Sürdürülebilir Kalkınma Amaçları
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| Google Scholar | 8 |
| Web of Science | 6 |