| Yazarlar (9) |
Ahmet Gozen
|
|
Seniz Demiryurek
|
|
Abdullah Taskin
|
|
Harun Ciralik
|
|
Hasan Bilinc
|
|
Sevgul Kara
|
Prof. Dr. Abdullah AYDIN
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Nurten Aksoy
|
|
Haluk Ceylan
|
| Özet |
| The aim of this study was to investigate the role of ischemic preconditioning (IPC) on ischemia/reperfusion (I/R)-induced injury of rat testis and determine the effects of 5-hydroxydecanoic acid (5-HD), a selective K(ATP) channel antagonist, and Y-27632, a selective Rho kinase inhibitor, on IPC. I/R injury was induced by 180 min ischemia and 60 min reperfusion of testis. There were 5 groups. Group 1 served as untreated controls. The rats in Group 2 were subjected to I/R only. In Group 3, 3 cycles of IPC (5 min transient ischemia plus 5 min reperfusion) were performed prior to I/R. In groups 4 and 5, the rats were treated as in Group 3 but received intraperitoneal injections of 0.3 mg/kg Y-27632 or 10 mg/kg 5-HD prior to IPC, respectively. I/R led to severe histopathological lesions in the rat testis and significantly lowered the scoring. I/R resulted in significant elevation in tissue lipid peroxide levels, myeloperoxidase (MPO) activity, and total antioxidative capacity (TAC), total oxidative status, and oxidative stress index levels. Protective effects of IPC on I/R-induced testicular injury of rats were observed with the significant recovery in these biochemical parameters. Y-27632 treatment led to a significant decrease in MPO activity, but there were no significant changes in the remaining parameters. Effects of IPC were blocked by 5-HD except in the TAC levels. Our results showed that IPC protected rat testis against I/R-induced injury via activation of KATP channels. Additionally, Rho kinase inhibition preserved the effects of IPC in testis. |
| Anahtar Kelimeler |
| Ischemic preconditioning | Testis | Y-27632 | 5-Hydroxydecanoic acid | Rho kinase |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | ESCI dergilerinde yayınlanan tam makale |
| Dergi Adı | JOURNAL OF PEDIATRIC SURGERY |
| Dergi ISSN | 0022-3468 Wos Dergi Scopus Dergi |
| Makale Dili | İngilizce |
| Basım Tarihi | 07-2013 |
| Cilt No | 48 |
| Sayı | 7 |
| Sayfalar | 1565 / 1572 |
| Doi Numarası | 10.1016/j.jpedsurg.2012.10.074 |
| Atıf Sayıları | |
| WoS | 21 |
