Development of a sample preparation and analysis method for therapeutic monitoring of diazepam and major metabolite in alcohol withdrawal syndrome treatment
Yazarlar (8)
Dr. Öğr. Üyesi Kenan Can Tok Ankara University, Türkiye
Doç. Dr. Selin ÖZKAN KOTİLOĞLU Kırşehir Ahi Evran Üniversitesi, Türkiye
H. Ceren Bozmaoğlu
Mustafa Danışman Ankara Training & Res Hosp AMATEM Clin
Prof. Dr. İnci İlhan Ankara Üniversitesi, Türkiye
Prof. Dr. Dilek Akyüzlü Ankara Üniversitesi, Türkiye
Prof. Dr. Halit Sinan Süzen Türkiye
Doç. Dr. Mehmet Gümüştaş Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (Q2) | ||
| Dergi ISSN | 0731-7085 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 08-2025 |
| Cilt / Sayı / Sayfa | 260 / 116805 / 116805–116805 | DOI | 10.1016/j.jpba.2025.116805 |
| Makale Linki | https://doi.org/10.1016/j.jpba.2025.116805 | ||
| Özet |
| Diazepam, a widely prescribed benzodiazepine, is frequently used for the management of alcohol withdrawal syndrome, anxiety, seizures, and muscle spasms. Its monitoring is critical due to its potential for abuse and the therapeutic importance of its metabolite nordiazepam. A sustainable and environmentally friendly high-performance liquid chromatography method was developed and validated for the quantification of diazepam and its active metabolite nordiazepam in human plasma samples. The chromatographic analysis was conducted on an HPLC system equipped with a UV detector. Separation was achieved using a Kinetex F5 column (150 ×4.6 mm, 5 µm). The mobile phase consisted of acetonitrile and phosphate buffer adjusted to pH 4.3 (30:70, v/v), delivered in isocratic mode at a flow rate of 1.0 mL/min, with the pH adjusted to optimize resolution. The method demonstrated excellent sensitivity … |
| Anahtar Kelimeler |
| Diazepam | Nordiazepam | Forensic Toxicology | HPLC-UV | Method Development and Validation | Drug Monitoring |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 4 |
| Google Scholar | 4 |