| Yazarlar (15) |
Emine Alarcin
Marmara Üniversitesi, Türkiye |
|
Zeynep Puren Akguner
İstinye Üniversitesi, Türkiye |
|
Ayca Bal Ozturk
İstinye Üniversitesi, Türkiye |
|
Gokcen Yasayan
Yeditepe University, Turkey |
Dr. Öğr. Üyesi Esra İLHAN AYIŞIĞI
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Aslihan Kazan
Bursa Teknik Üniversitesi, Turkey |
|
Ozlem Yesil-Celiktas
Ege University Faculty of Engineering, Turkey |
|
Dila Sener Akcora
Marmara Üniversitesi Tip Fakültesi, Türkiye |
|
Dilek Akakin
Marmara Üniversitesi Tip Fakültesi, Türkiye |
|
Banu Kocaaga
İstanbul Teknik Üniversitesi, Turkey |
|
Gamze Eren
Private Clinic, Turkey |
|
Kasım Gunes
Marmara Üniversitesi Tip Fakültesi, Türkiye |
|
Oya Kerimoglu
Marmara Üniversitesi, Türkiye |
|
Hatice Kubra Seki
Yeditepe University, Turkey |
|
F. Seniha Guner
İstanbul Teknik Üniversitesi, Turkey |
| Özet |
| The effective treatment of critical-sized bone defects requires a coordinated interaction between osteogenesis and angiogenesis. Inspired by natural bone tissue, we developed a bilayer vascularized bone construct using extrusion-based dual 3D bioprinting. The construct consists of two layers: a bone-mimetic layer, which includes highly methacrylated gelatin (GelMAHIGH), hyaluronic acid, alginate, osteoblast cells, and bone morphogenetic protein-2 (BMP-2) loaded polylactic-co-glycolic acid (PLGA) nanoparticles; and a vessel-mimetic layer, composed of low methacrylated gelatin (GelMALOW), alginate, endothelial cells, and vascular endothelial growth factor (VEGF)-loaded PLGA nanoparticles. These layers were designed to form hierarchical microstructures that enable sustained release of growth factor (GF) thereby stimulating both osteogenic and angiogenic processes. The nanoparticles were synthesized using a microfluidic platform, achieving a narrow size distribution. The hydrogel bioinks were systematically optimized for printability, and it was found that incorporation of nanoparticles improved their mechanical properties, surface roughness, degradability, and GF release profiles. Notably, GF release followed zero-order kinetics, ensuring consistent delivery over time. The bilayer scaffolds demonstrated superior cell proliferation and spreading compared to single-layer scaffolds, and in vivo experiments showed enhanced repair of calvarial bone defects. These findings highlight the significant clinical potential of bilayer scaffolds with sequential GF delivery for treating critical-sized bone defects. |
| Anahtar Kelimeler |
| 3D bioprinting | Bilayer scaffold | Bone regeneration | Bone scaffold | Controlled growth factor release | Vascularization |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | International Journal of Biological Macromolecules |
| Dergi ISSN | 0141-8130 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2025 |
| Cilt No | 306 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.ijbiomac.2025.141440 |
| Atıf Sayıları |
