| Yazarlar (1) |
Dr. Öğr. Üyesi Ayşe Nur COŞKUN DEMİRKALP
Kırşehir Ahi Evran Üniversitesi, Türkiye |
| Özet |
| Toxicology tests are one of the fundamental methods used in biological and pharmaceutical research. These tests are used to evaluate the viability, proliferation and toxic responses of cells. The study of biological activities of cells plays a critical role in drug development, cancer research, toxicology and various biotechnological applications. Drug toxicology is an important field of research to determine the harmful effects of drugs on cellular and biological systems. In vitro tests are widely used for accurate evaluation of drugs and their toxic effects. These tests examine the effects of drugs on cell cultures, rapidly revealing their potential harmful effects in terms of time and resources. This review discusses the advantages of the MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) and XTT (sodium 3'-[1-(phenylamino)-carbonyl]-3,4-tetrazolium]-bis (4 methoxy-6-nitro) benzene-sulfonic acid hydrate) tests, which are widely used as cytotoxicity tests, as well as the newer method iCELLigence. MTT and XTT are widely used and reliable tests that measure cell metabolism; both methods are very effective in assessing cell viability, but provide limited dynamic data. In contrast, iCELLigence is a newer technology and provides more in-depth data by monitoring the real-time responses of cells. iCELLigence continuously monitors the growth rate and morphological changes of cells, allowing for more comprehensive and sensitive results compared to traditional methods. Comparison of these methods allows determining which methodology provides more appropriate results according to different research needs.These tests are also used to define … |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale |
| Dergi Adı | Journal of Medicine and Palliative Care |
| Dergi ISSN | 2717‑7505 |
| Dergi Tarandığı Indeksler | TR DİZİN |
| Makale Dili | Türkçe |
| Basım Tarihi | 02-2025 |
| Cilt No | 6 |
| Sayı | 1 |
| Sayfalar | 66 / 71 |
| Doi Numarası | 10.47582/jompac.1605042 |
| Makale Linki | https://doi.org/10.47582/jompac.1605042 |
| Atıf Sayıları | |
| Google Scholar | 1 |
