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Molecular Characterization of Gram Negative Isolated from Walnut (Juglans regia L.) Rhizosphere     
Yazarlar
Prof. Dr. Belgin ERDEM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Elif SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Prof. Dr. Ali SEVİM
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
In this study, 10 Gram negative bacteria isolated from the rhizosphere soil of walnut (Juglans regia) orchard in the Kırşehir (Central Anatolia of Turkey) were identified at species level by classical and molecular methods. It was determined that 99% of phylogenetic similarities with bacterial isolates were closely related to the isolated Gram negative bacterial isolates. Ten isolates were morpho-biochemically and molecularly characterized and on the basis of 16S rDNA sequencing were identified as 3 Enterobacter cloacae, 3 Enterobacter ludwigii, 2 Escherichia coli, 1 Enterobacter hormaechei and 1 Plesiomonas shigelloides. Gram negative bacteria showed enzymes activities. Except for P. shigelloides and E. coli, other Gram negative bacteria showed lipase, protease, cellulase, amylase, gelatinase and pectinase activities at 4 °C and 28 °C. As a result, the current research suggests that strains isolated from walnut rhizosphere soil can be used as bio-fertilizing and bio-control agents.
Anahtar Kelimeler
Gram negetive bacteria,16S rRNA sequence,Juglans regia L.,Rhizosphere
Makale Türü Özgün Makale
Makale Alt Türü Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayımlanan tam makale
Dergi Adı Black Sea Journal of Engineering and Science
Dergi ISSN e-ISSN: 2619 –
Dergi Tarandığı Indeksler TR DİZİN
Makale Dili İngilizce
Basım Tarihi 04-2022
Cilt No 5
Sayı 2
Sayfalar 54 / 61
Doi Numarası 10.34248/bsengineering.957791
Makale Linki 10.34248/bsengineering.957791