| Yazarlar (5) |
Sabriye BELGÜZAR
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|
Melih YILAR
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|
Yusuf YANAR
|
|
İzzet KADIOĞLU
|
|
Gizem DOĞAR
|
| Özet |
| This study was performed to determine antibacterial effects of Thyme (Thymus vulgaris L.), that collected Mersin, aqueous extract and essential oil against Clavibacter michiganensis subsp. michiganensis under in vitro conditions. Aqueous extract of the plant was adcusted to get final concentrations of 0.5%, 1%, 2%, 4%, 8% than were added Pseudomonas Agar F (PSF) medium. 106 cells/ml of C. michiganensis subsp. michiganensis suspansion was plated on the medium containing the extract. In antibacterial activity tests, three different method were used for application of thyme essential oil, mixing in medium, filter paper impregnation and well diffision methods. Four different dose (5, 10, 15 and 20 µl/ml) of essential oil were tested. The plain PSF medium was used as a control in each test. Experiment was set up with four replications and repeated twice. At the end of incubation period (28°C for 3 days), colony count in extract study, and measurement of inhibition zone and colony count in essential oil study was performed. According to the results, colony number of C. michiganensis subsp. michiganensis was decreased with increase in dose of T. vulgaris extract and 8% extract concentration resulted in 100% colony growth inhibition. In essential oil study, filter paper impregnation was the most effective method and 20 µl/ml essential oil of T. vulgaris inhibited 99.39% colony growth of C. michiganensis subsp. michiganensis. In conclusion, thymus extract and essential oil have potential to use as antibacterial agents. Further studies should be performed to determine the invitro efficacies and phytoticic effects of thymus extract and essential oil |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Uluslararası alan indekslerindeki dergilerde yayımlanan tam makale |
| Dergi Adı | Turkish Journal of Weed Science |
| Dergi Tarandığı Indeksler | |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2016 |
| Cilt No | 19 |
| Sayı | 2 |
| Sayfalar | 20 / 27 |
