| Yazarlar |
Cem Dirlik
|
|
Hacer Kandemir
|
|
Nurberat Çetin
|
|
Senem Şen
|
Dr. Öğr. Üyesi Begüm GÜLER
Kırşehir Ahi Evran Üniversitesi, Türkiye |
|
Aynur Gürel
Ege Üniversitesi, Türkiye |
| Özet |
| The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the 'Paradox' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayımlanan tam makale |
| Dergi Adı | Gazi University |
| Dergi ISSN | 2147-9542 |
| Dergi Tarandığı Indeksler | “TUBITAK ULAKBIM, TR Dizin, Index Copernicus (ICI), SOBIAD, Directory of Research Journals Indexing (DRJI), Academic Resource Index (RESEARCHBIB), ASOS Index (ASOS), Scientific Indexing Services, Info Base Index, Google Scholar |
| Makale Dili | İngilizce |
| Basım Tarihi | 12-2022 |
| Cilt No | 9 |
| Sayı | 4 |
| Sayfalar | 500 / 515 |
| Doi Numarası | 10.54287/gujsa.1194822 |
| Makale Linki | http://dx.doi.org/10.54287/gujsa.1194822 |
| Atıf Sayıları | |
| TRDizin | 1 |
