Assessment of the Effects of Two Different Doses of Methotrexate in Rat Ovarian Tissue: A Histological Study
Yazarlar (5)
Betül Yalçın
Türkiye
Türkiye
Dr. Öğr. Üyesi Kübra Tuğçe KALKAN Kırşehir Ahi Evran Üniversitesi, Türkiye
Eda Okur Erciyes Üniversitesi, Türkiye
Enes Karaman
Niğde Ömer Halisdemir Üniversitesi, Türkiye
Niğde Ömer Halisdemir Üniversitesi, Türkiye
Arzu Hanım Yay Erciyes Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale)
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| Dergi Adı | Annals of Medical Research | ||
| Dergi ISSN | 2636-7688 | ||
| Dergi Tarandığı Indeksler | TR DİZİN | ||
| Makale Dili | İngilizce | Basım Tarihi | 08-2024 |
| Cilt / Sayı / Sayfa | 31 / 8 / 582–586 | DOI | 10.5455/annalsmedres.2024.05.098 |
| Makale Linki | https://doi.org/10.5455/annalsmedres.2024.05.098 | ||
| Özet |
| Aim: The use of methotrexate (MTH), a drug commonly prescribed to treat rheumatoid arthritis, cancer, and certain autoimmune diseases, has been linked to early ovarian failure and infertility in women when used in varying amounts and durations. In this study, the possible effects of MTH exposure at different doses on rat ovary were investigated histologically and immunohistochemically.Materials and Methods: A total of 21 female rats, Wistar albino, were utilized in the investigation. The control group (n= 7) was not subjected to any treatment. The 10 mg/kg MTH group (n= 7) and the 20 mg/kg MTH group (n= 7) were given intraperitoneal injections of 10 mg/kg and 20 mg/kg methotrexate, respectively. Five days after injection, the ovarian tissues were taken out of the anesthetized rats and subjected to routine histological tissue processing. The histological evaluation was performed with hematoxylin and eosin (H&E) and Masson's trichrome (MT) on paraffin block sections. Additionally, immunohistochemical staining was performed to determine AMH immunoreactivity.Results: In both MTH groups, histopathological changes were observed, particularly in the 20 mg/kg group, and these included the presence of areas of edema as well as dilated or congested blood vessels. Furthermore, the 20 mg/kg MTH group exhibited increased fibrotic tissue in the ovarian medulla. The number of follicles and AMH immunoreactivity were observed to be reduced in the MTH groups, with the reduction reaching a statistically significant level in the 20 mg/kg MTH group ovary. In terms of AMH immunoreactivity, it is demonstrated that there is no statistical significance … |
| Anahtar Kelimeler |
| Anti-Mullerian hormone | Methotrexate | Ovarian reserve |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 2 |