| Yazarlar (2) |
Arş. Gör. Ömer Yusuf İPEK
Kırşehir Ahi Evran Üniversitesi, Türkiye |
Marco Canepari
|
| Özet |
| Calcium imaging is a valuable technique in neuroscience, as changes in calcium ion concentration provide crucial insights into neural activity. However, it is limited by photo-bleaching, photo-toxicity, and high noise levels, which complicate achieving high-quality spatiotemporal resolution (Robbins et al., 2021). We aimed to study rapid neural activity within the hippocampus using calcium imaging. To this end, we employed a novel camera (Kinetix Scientific CMOS) capable of recording at up to 80 kHz with 8-bit resolution. Hippocampal brain slices from mice (~ 3rd postnatal week) were dyed with Fluo-4, a Ca2+ indicator, to identify calcium signals, and bicuculline was used to induce visible network activity following direct stimulation on the slice. We developed MATLAB scripts for photobleaching correction and signal strength enhancement through filtering. Our results demonstrate that 8-bit recordings provide … |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | 9th International Brain Research School / Journal of Cellular Neuroscience & Oxidative Stress, 2024 |
| Kongre Tarihi | 20-05-2024 / 26-05-2024 |
| Basıldığı Ülke | Türkiye |
| Basıldığı Şehir | Isparta |
