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Biochemical Responses of Sainfoin (Onobrychis viciifolia Scop.) Callus Tissue to Low Dose Gamma Radiation   
Yazarlar (1)
Prof. Dr. Ramazan BEYAZ Prof. Dr. Ramazan BEYAZ
Kırşehir Ahi Evran Üniversitesi, Türkiye
Devamını Göster
Özet
In this study, biochemical changes in callus tissue caused by low dose (50 Gray) gamma radiation were investigated. For this purpose, seedlings were developed in vitro from seeds exposed to gamma radiation. The petioles of these seedlings were used as explants for callus formation. Biochemical parameters such as antioxidative enzyme activities (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR)), malondialdehyde (MDA) and proline content were measured in 30-day callus tissue. As a result of the study, it was determined that the activities of antioxidative enzymes (except for APX), malondialdehyde and proline content were statistically increased in callus tissues. Considering the increase rate in callus tissues, it was determined that the highest increase in biochemical parameters was in SOD activity and proline content. The importance of this study is to show that irradiation of seeds with gamma radiation is sufficient to change the biochemical content of callus tissues obtained from any plant part under in vitro conditions. As a result, a plant breeders who uses biotechnological approaches in their studies can easily benefit from the evidence presented in this study.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Diğer hakemli ulusal dergilerde yayınlanan tam makale
Dergi Adı KIRŞEHİR AHİ EVRAN ÜNİVERSİTESİ ZİRAAT FAKÜLTESİ DERGİSİ
Dergi ISSN 2791-9161
Dergi Tarandığı Indeksler Alan endeksleri
Makale Dili İngilizce
Basım Tarihi 10-2021
Cilt No 1
Sayı 1
Sayfalar 21 / 30
Makale Linki https://dergipark.org.tr/tr/pub/kuzfad/issue/68421/1226211
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