img
Effect of DGAT1 gene polymorphisms in coarse-haired fat-tailed lambs of different genotypes       
Yazarlar
Arş. Gör. Fatih ÖZYURT Arş. Gör. Fatih ÖZYURT
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
The aim of this study was to investigate the DGAT1 gene polymorphism and its effects on lamb weight in kazakh and tajik sheep breeds. A total of 97 blood samples were collected from purebred (edilbay × edilbay) and crossbred lambs (edilbay × gissar) breed by the Baiserke Agro Scientific and Production Center in the Talgar District of the Almaty Region of Kazakhstan. Animals were genotyped for DGAT1-AluI polymorphism using the polymerase chain reaction-restriction length polymorphism (PCR-RFLP) method. The result of PCR-RFLP showed that purebred (edilbay × edilbay) sheep had three genotypes (CC, CT and TT) and crossbred sheep had two genotypes (CC and CT). The predominant genotype was CC with a frequency of 0.70 and 0.58 in purebred sheep and crossbred sheep breeds, respectively. The DGAT1 gene showed no significant association with live weight of lambs at different times in both breeds studied. However, the study showed that the CC genotype produced higher live weight at day 60 in purebred sheep (CC: 33,668 kg and CT: 32,444) and at day 120 (CC: 41,487 and CT: 40,929) in crossbred lambs. The present study was the first to investigate the polymorphism and relationships between genotypes and lamb live weights for DGAT1 gene in sheep breeds, purebred and crossbred. We conclude that further comprehensive investigations should be done for the exact evidence of the effects of DGAT1/Aluı polymorphism on lamb live weights.
Anahtar Kelimeler
AluI | crossbred | DGAT1 gene | Edilbay | gissar | lamb weight | PCR-RFL | purebred
Makale Türü Diğer (Teknik, not, yorum, vaka takdimi, editöre mektup, özet, kitap krıtiği, araştırma notu, bilirkişi raporu ve benzeri)
Makale Alt Türü Diğer hakemli ulusal dergilerde yayımlanan teknik not, editöre mektup, tartışma, vaka takdimi ve özet türünden makale
Dergi Adı Brazilian Journal of Biology
Dergi ISSN 1519-6984
Dergi Tarandığı Indeksler Scopus
Makale Dili Türkçe
Basım Tarihi 01-2024
Cilt No 84
Sayı 1
Sayfalar 1 / 5
Doi Numarası 10.1590/1519-6984.285041
Makale Linki http://dx.doi.org/10.1590/1519-6984.285041