| Yazarlar (1) |
Ramazan Beyaz
Kırşehir Ahi Evran Üniversitesi |
| Özet |
| Effective methods for gene silencing have shown a remarkable increase in the field of functional genomics (gene function), as sequencing analyzes of many model organisms reveal a large number of genes whose function is not yet known. Numerous gene silencing mechanisms have emerged over the years, primarily targeting the genes themselves or the mRNAs they encode. Antisense strategies are widely used in these mRNA-targeted techniques. These antisense strategies rely entirely on delivering a DNA or RNA nucleic acid strand into cells that is reverse complementary to the mRNA encoding the targeted protein.Antisense oligonucleotide studies have led to the discovery of RNA interference (RNAi), a highly effective method for gene silencing in recent years. RNA interference is a post-transcriptional gene silencing mechanism that results in the fragmentation of the complementary mRNA sequence when double-stranded RNA (dsRNA) enters the cell. This mechanism is a natural process and its biological function in living organisms is to play a role in cellular defense by protecting the genome against the invasion of mobile genetic elements such as virus hereditary material and transposons. It also plays an important role in gene regulation by post-transcriptional gene silencing, which is important for the function of the developmental programs of eukaryotic organisms. Because RNAi is a naturally occurring pathway, the efficacy and specificity of this technique differs from other nucleic acid-based silencing/repression techniques such as antisense oligonucleotide and ribozymes. Thus, RNAi has emerged as an important potential … |
| Anahtar Kelimeler |
| Kitap Adı | FUNCTIONAL GENE SILENCE TECHNIQUE IN PLANTS: VIRUS-INDUCED GENE SILENCING (VIGS) |
| Kitap Türü | Kitap Bölümü |
| Kitap Alt Türü | Alanında uluslararası yayınlanan kitap bölümü |
| Kitap Niteliği | Diğer uluslararası bilimsel kitap |
| Kitap Dili | İngilizce |
| Basım Tarihi | 08-2023 |
| ISBN | 978-625-367-194-5 |
| Basıldığı Ülke | Türkiye |
| Basıldığı Şehir |
