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Comparison of molecular hybridization, real time PCR and classical PCR techniques for diagnosis of GYSVD-1 (Grapevine yellow speckle viroid 1)    
Yazarlar (5)
İsmail Can Paylan
Ege Üniversitesi, Türkiye
Dr. Öğr. Üyesi Ayşe ÇANDAR Dr. Öğr. Üyesi Ayşe ÇANDAR
Kırşehir Ahi Evran Üniversitesi, Türkiye
Serkan Önder
Eskişehir Osmangazi Üniversitesi, Türkiye
Nihan Güneş
Ege Üniversitesi, Türkiye
Mustafa Gümüş
Ege Üniversitesi, Türkiye
Devamını Göster
Özet
Viroids are the smallest known plant pathogens and have been identified as agents of several economically significant crop diseases. They comprise small (246–401 nt), uncapsidated, covalently closed circular, non-coding RNA molecules which rely entirely on host factors for their replication (Flores et al. 2005; Ding 2009; Zhang et al. 2011). Grapevine yellow speckle viroid 1 (GYVSd-1) is a viroid in the family Pospiviroidae. GYSVd-1 is found in the important production areas, is very easily spread mechanically by contaminated cutting tools (Szychowski et al., 1988), infected graft (Szychowski et al., 1988; Staub et al., 1995), propagation materials (Kultonow et al., 1988) and causes severe damage to quality and quantity of grapevines. Rapid diagnosis of viroid infections may be obtained by bioassays, polyacrylamide gel electrophoresis (PAGE), molecular techniques such as polymerase chain reaction (PCR) and hybridization assays. In this study we aimed to compare three molecular methods including molecular hybridization, real-time pcr and classical pcr in order to detect GYSVd-1.
Anahtar Kelimeler
Bildiri Türü Tebliğ/Bildiri
Bildiri Alt Türü Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum)
Bildiri Niteliği Alanında Hakemli Uluslararası Kongre/Sempozyum
Bildiri Dili İngilizce
Kongre Adı 18th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG) , Ankara, Turkey, 7-11 September 2015.
Kongre Tarihi 07-09-2015 / 11-09-2015
Basıldığı Ülke Türkiye
Basıldığı Şehir Ankara
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları

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