| Yazarlar (5) |
İsmail Can Paylan
Ege Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Ayşe ÇANDAR
|
|
Serkan Önder
Eskişehir Osmangazi Üniversitesi, Türkiye |
|
Nihan Güneş
Ege Üniversitesi, Türkiye |
|
Mustafa Gümüş
Ege Üniversitesi, Türkiye |
| Özet |
| Viroids are the smallest known plant pathogens and have been identified as agents of several economically significant crop diseases. They comprise small (246–401 nt), uncapsidated, covalently closed circular, non-coding RNA molecules which rely entirely on host factors for their replication (Flores et al. 2005; Ding 2009; Zhang et al. 2011). Grapevine yellow speckle viroid 1 (GYVSd-1) is a viroid in the family Pospiviroidae. GYSVd-1 is found in the important production areas, is very easily spread mechanically by contaminated cutting tools (Szychowski et al., 1988), infected graft (Szychowski et al., 1988; Staub et al., 1995), propagation materials (Kultonow et al., 1988) and causes severe damage to quality and quantity of grapevines. Rapid diagnosis of viroid infections may be obtained by bioassays, polyacrylamide gel electrophoresis (PAGE), molecular techniques such as polymerase chain reaction (PCR) and hybridization assays. In this study we aimed to compare three molecular methods including molecular hybridization, real-time pcr and classical pcr in order to detect GYSVd-1. |
| Anahtar Kelimeler |
| Bildiri Türü | Tebliğ/Bildiri |
| Bildiri Alt Türü | Özet Metin Olarak Yayınlanan Tebliğ (Uluslararası Kongre/Sempozyum) |
| Bildiri Niteliği | Alanında Hakemli Uluslararası Kongre/Sempozyum |
| Bildiri Dili | İngilizce |
| Kongre Adı | 18th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG) , Ankara, Turkey, 7-11 September 2015. |
| Kongre Tarihi | 07-09-2015 / 11-09-2015 |
| Basıldığı Ülke | Türkiye |
| Basıldığı Şehir | Ankara |
