Comparison of Real-time PCR, RT-PCR and Molecular Hybridization Techniques for the Diagnosis of GYSVD-1 (Grapevine Yellow Speckle Viroid-1) in Grapevines
Yazarlar (6)
Prof. Dr. İsmail Can Paylan Ege University, Malezya
Dr. Öğr. Üyesi Ayşe ÇANDAR Kırşehir Ahi Evran Üniversitesi, Türkiye
Dr. Öğr. Üyesi Serkan Önder Plant Hlth Dept, Türkiye
Nihan Güneş Ege Üniversitesi, Türkiye
Mustafa Gümüş Ege Üniversitesi, Türkiye
Semih Erkan Ege University, Malezya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | FRESENIUS ENVIRONMENTAL BULLETIN (Q4) | ||
| Dergi ISSN | 1018-4619 Dergi Bilgileri (2018) | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2018 |
| Cilt / Sayı / Sayfa | 27 / 5 / 3326–3331 | DOI | 10.5555/20183253199 |
| Makale Linki | https://www.researchgate.net/publication/328830319_Comparison_of_Real-time_PCR_RT-PCR_and_Molecular_Hybridization_Techniques_for_the_Diagnosis_of_GYSVD-1_Grapevine_Yellow_Speckle_Viroid-1_in_Grapevines | ||
| UAK Araştırma Alanları |
Fitopatoloji
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| Özet |
| GYSVd-1 is very easily spread mechanically by contaminated cutting tools and infected graft propagation materials and reduces the quality and quantity of grapevines. Rapid diagnosis of viroid infections can be obtained by bioassays, polyacrylamide gel electrophoresis (PAGE), molecular techniques such as polymerase chain reaction (PCR) and hybridization assays. In this study, we aimed to compare three molecular methods including molecular hybridization, real-time PCR and RT-PCR in order to detect GYSVd-1 in grapevines. In the diagnostic analyses conducted with 50 potentially positive grapevine samples, GYSVd-1 was detected in 45 of 50 samples by one or more detection techniques. 18 samples were found to be positive by all methods. 18 other samples gave positive results in real-time PCR and RT-PCR analyses but were negative in molecular hybridization tests. Two samples were positively … |
| Anahtar Kelimeler |
| GYSVd-1 | Real-time PCR | RT-PCR | Molecular Hybridization | Grapevine | Viroid |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 1 |