img
Use of random amplified polymorphic DNA (RAPD) to detect DNA damage induced by Prangos ferulacea (Umbelliferae) essential oil against the Mediterranean flour moth Ephestia ...  
Yazarlar
Prof. Dr. Fahriye ERCAN
Kırşehir Ahi Evran Üniversitesi, Türkiye
Özet
DNA damage detection in Ephestia kuehniella by RAPD-PCR Abstract - The random amplified polymorphic DNA (RAPD) assay is a useful method for detecting genotoxin-induced DNA damage. In the present study, this assay was evaluated to measure the essential oil of Prangos ferulacea-induced DNA changes in Ephestia kuehniella Zeller larvae. The third instar larvae of E. kuehniella were exposed to 500 μl L-1 essential oil for 24 h. Forty random primers were used for RAPD-PCR of which eleven produced unique polymorphic band patterns. In comparison with control larvae, the essential oil-treated larvae showed greater changes in RAPD profiles. This is the first report of an analysis of the genotoxic effect of P. ferulacea essential oil against E. kuehniella larvae using RAPD-PCR.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Archives of Biological Sciences
Dergi Tarandığı Indeksler
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2015
Cilt No 67
Sayı 1
Sayfalar 235 / 239
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Google Scholar 14

Paylaş